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Hypoxia in the thymus: role of oxygen tension in thymocyte survival.

Publication ,  Journal Article
Hale, LP; Braun, RD; Gwinn, WM; Greer, PK; Dewhirst, MW
Published in: Am J Physiol Heart Circ Physiol
April 2002

Our previous studies using oxygen microelectrodes showed that the thymus is grossly hypoxic under normal physiological conditions. We now have investigated how oxygen tension affects the thymus at the cellular and molecular level. Adducts of the hypoxia marker drug pimonidazole accumulated in foci within the cortex and medulla and at the corticomedullary junction, consistent with the presence of widespread cellular hypoxia in the normal thymus. Hypoxia-associated pimonidazole accumulation was decreased but not abrogated by oxygen administration. Genes previously reported to be induced by hypoxia were expressed at baseline levels in the normal thymus, indicating that physiological adaptation to hypoxia occurred. Despite changes in thymus size and cellularity, thymic PO(2) did not change with age. Combined assays for hypoxia and cell death showed that hypoxia achieved using either hypoxic gas mixtures or high-density culture in normoxia decreased spontaneous thymocyte apoptosis in vitro. Taken together, these data suggest that regulatory mechanisms exist to maintain thymic cellular hypoxia in vivo and that oxygen tension may regulate thymocyte survival both in vitro and in vivo.

Duke Scholars

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

April 2002

Volume

282

Issue

4

Start / End Page

H1467 / H1477

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Thymus Gland
  • Radiation-Sensitizing Agents
  • RNA, Messenger
  • Prostaglandin-Endoperoxide Synthases
  • Polymerase Chain Reaction
  • Partial Pressure
  • Oxygen Consumption
  • Oxygen
  • Nitroimidazoles
 

Citation

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Chicago
ICMJE
MLA
NLM
Hale, L. P., Braun, R. D., Gwinn, W. M., Greer, P. K., & Dewhirst, M. W. (2002). Hypoxia in the thymus: role of oxygen tension in thymocyte survival. Am J Physiol Heart Circ Physiol, 282(4), H1467–H1477. https://doi.org/10.1152/ajpheart.00682.2001
Hale, Laura P., Rod D. Braun, William M. Gwinn, Paula K. Greer, and Mark W. Dewhirst. “Hypoxia in the thymus: role of oxygen tension in thymocyte survival.Am J Physiol Heart Circ Physiol 282, no. 4 (April 2002): H1467–77. https://doi.org/10.1152/ajpheart.00682.2001.
Hale LP, Braun RD, Gwinn WM, Greer PK, Dewhirst MW. Hypoxia in the thymus: role of oxygen tension in thymocyte survival. Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1467–77.
Hale, Laura P., et al. “Hypoxia in the thymus: role of oxygen tension in thymocyte survival.Am J Physiol Heart Circ Physiol, vol. 282, no. 4, Apr. 2002, pp. H1467–77. Pubmed, doi:10.1152/ajpheart.00682.2001.
Hale LP, Braun RD, Gwinn WM, Greer PK, Dewhirst MW. Hypoxia in the thymus: role of oxygen tension in thymocyte survival. Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1467–H1477.

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

April 2002

Volume

282

Issue

4

Start / End Page

H1467 / H1477

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Thymus Gland
  • Radiation-Sensitizing Agents
  • RNA, Messenger
  • Prostaglandin-Endoperoxide Synthases
  • Polymerase Chain Reaction
  • Partial Pressure
  • Oxygen Consumption
  • Oxygen
  • Nitroimidazoles