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Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft.

Publication ,  Journal Article
Friedman, HS; Johnson, SP; Dong, Q; Schold, SC; Rasheed, BK; Bigner, SH; Ali-Osman, F; Dolan, E; Colvin, OM; Houghton, P; Germain, G; Keir, S ...
Published in: Cancer Res
July 15, 1997
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A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)-1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.

Duke Scholars

Henry Seth Friedman
Author Henry Seth Friedman Neurosurgery, Neuro-Oncology
Francis Ali-Osman
Author Francis Ali-Osman Neurosurgery, Neuro-Oncology
Stephen Thomas Keir
Author Stephen Thomas Keir Neurosurgery, Neuro-Oncology
Darell Doty Bigner
Author Darell Doty Bigner Neurosurgery
Paul L. Modrich
Author Paul L. Modrich Biochemistry

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 15, 1997

Volume

57

Issue

14

Start / End Page

2933 / 2936

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Oncology & Carcinogenesis
  • O(6)-Methylguanine-DNA Methyltransferase
  • Neoplasm Transplantation
  • Microsatellite Repeats
  • Mice, Inbred BALB C
  • Mice
  • Methyltransferases
  • Melanocyte-Stimulating Hormones
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Friedman, H. S., Johnson, S. P., Dong, Q., Schold, S. C., Rasheed, B. K., Bigner, S. H., … Modrich, P. (1997). Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft. Cancer Res, 57(14), 2933–2936.
Friedman, H. S., S. P. Johnson, Q. Dong, S. C. Schold, B. K. Rasheed, S. H. Bigner, F. Ali-Osman, et al. “Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft.” Cancer Res 57, no. 14 (July 15, 1997): 2933–36.
Friedman HS, Johnson SP, Dong Q, Schold SC, Rasheed BK, Bigner SH, et al. Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft. Cancer Res. 1997 Jul 15;57(14):2933–6.
Friedman, H. S., et al. “Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft.” Cancer Res, vol. 57, no. 14, July 1997, pp. 2933–36.
Friedman HS, Johnson SP, Dong Q, Schold SC, Rasheed BK, Bigner SH, Ali-Osman F, Dolan E, Colvin OM, Houghton P, Germain G, Drummond JT, Keir S, Marcelli S, Bigner DD, Modrich P. Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft. Cancer Res. 1997 Jul 15;57(14):2933–2936.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

July 15, 1997

Volume

57

Issue

14

Start / End Page

2933 / 2936

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Oncology & Carcinogenesis
  • O(6)-Methylguanine-DNA Methyltransferase
  • Neoplasm Transplantation
  • Microsatellite Repeats
  • Mice, Inbred BALB C
  • Mice
  • Methyltransferases
  • Melanocyte-Stimulating Hormones