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Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis.

Publication ,  Journal Article
Welty-Wolf, KE; Carraway, MS; Huang, YC; Simonson, SG; Kantrow, SP; Kishimoto, TK; Piantadosi, CA
Published in: Am J Respir Crit Care Med
March 2001

Neutrophil influx into the lung is an important event in the pathogenesis of acute lung injury in gram-negative sepsis. We hypothesized that administration of a monoclonal antibody to intercellular adhesion molecule 1 (ICAM-1, CD54), a molecule mediating neutrophil adhesion to endothelial cells, would decrease neutrophil sequestration and transmigration in the lung and attenuate lung injury in Escherichia coli sepsis. Sepsis was induced in 12 baboons primed with heat-killed E. coli (1 x 10(9) CFU/kg) 12 h before infusion of live bacteria (1 x 10(10) CFU/kg). Six animals received monoclonal antibody to CD54 (1 mg/kg) intravenously at the time of live E. coli infusion. After 48 h or when blood pressure could not be maintained, tissues were harvested and bronchoalveolar lavage (BAL) samples were obtained. Median survival time was decreased in anti-CD54-treated animals. This group also had decreased mean arterial pressure, increased metabolic acidosis, and decreased urine output. Measures of lung injury including gas exchange, lung lavage protein and lactate dehydrogenase (LDH), lung thiobarbituric acid-reactive species, and lung histology, including alveolar neutrophil volumes, were unaffected by treatment. The effect of anti-CD54 on neutrophil influx into tissues as measured by myeloperoxidase was organ specific. These data show that monoclonal antibody to CD54 does not ameliorate acute lung injury in E. coli sepsis, and septic primates given anti-CD54 have worsened metabolic parameters and decreased survival.

Duke Scholars

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

March 2001

Volume

163

Issue

3 Pt 1

Start / End Page

665 / 673

Location

United States

Related Subject Headings

  • Survival Rate
  • Sepsis
  • Respiratory System
  • Respiratory Distress Syndrome
  • Papio
  • Male
  • Intercellular Adhesion Molecule-1
  • Hemodynamics
  • Antibodies, Monoclonal
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Welty-Wolf, K. E., Carraway, M. S., Huang, Y. C., Simonson, S. G., Kantrow, S. P., Kishimoto, T. K., & Piantadosi, C. A. (2001). Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis. Am J Respir Crit Care Med, 163(3 Pt 1), 665–673. https://doi.org/10.1164/ajrccm.163.3.2004191
Welty-Wolf, K. E., M. S. Carraway, Y. C. Huang, S. G. Simonson, S. P. Kantrow, T. K. Kishimoto, and C. A. Piantadosi. “Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis.Am J Respir Crit Care Med 163, no. 3 Pt 1 (March 2001): 665–73. https://doi.org/10.1164/ajrccm.163.3.2004191.
Welty-Wolf KE, Carraway MS, Huang YC, Simonson SG, Kantrow SP, Kishimoto TK, et al. Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis. Am J Respir Crit Care Med. 2001 Mar;163(3 Pt 1):665–73.
Welty-Wolf, K. E., et al. “Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis.Am J Respir Crit Care Med, vol. 163, no. 3 Pt 1, Mar. 2001, pp. 665–73. Pubmed, doi:10.1164/ajrccm.163.3.2004191.
Welty-Wolf KE, Carraway MS, Huang YC, Simonson SG, Kantrow SP, Kishimoto TK, Piantadosi CA. Antibody to intercellular adhesion molecule 1 (CD54) decreases survival and not lung injury in baboons with sepsis. Am J Respir Crit Care Med. 2001 Mar;163(3 Pt 1):665–673.

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

March 2001

Volume

163

Issue

3 Pt 1

Start / End Page

665 / 673

Location

United States

Related Subject Headings

  • Survival Rate
  • Sepsis
  • Respiratory System
  • Respiratory Distress Syndrome
  • Papio
  • Male
  • Intercellular Adhesion Molecule-1
  • Hemodynamics
  • Antibodies, Monoclonal
  • Animals