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Hepatocyte receptors for antithrombin III-proteinase complexes.

Publication ,  Journal Article
Fuchs, HE; Shifman, MA; Michalopoulos, G; Pizzo, SV
Published in: J Cell Biochem
1984

The in vivo clearance of antithrombin III-proteinase complexes occurs via a specific and saturable pathway located on hepatocytes. We now report studies of the catabolism of antithrombin III-proteinase complexes in vitro using rat hepatocytes in primary culture. Antithrombin III-thrombin and trypsin complexes were prepared and purified to homogeneity. Ligand uptake by hepatocytes was concentration, temperature, and time dependent. Initial rate studies were performed to characterize the maximum rate of uptake, V, and apparent Michaelis constant Kapp. These studies yielded a V of 12.8 fmol/mg cell protein/min and a Kapp of 144 nM for antithrombin-trypsin complexes. Competition experiments with antithrombin III, antithrombin III-proteinase complexes, alpha 2-macroglobulin-methylamine, asialoorosomucoid and the neoglycoproteins, fucosyl-bovine serum albumin (BSA), N-acetylglucosaminyl-BSA, and mannosyl-BSA indicated that only antithrombin III-proteinase complexes were recognized by the hepatocyte receptor. Uptake studies were performed at 37 degrees C with 125I-antithrombin III-trypsin and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in conjunction with autoradiography. These studies demonstrate time-dependent uptake and degradation of the ligand to low molecular weight peptides. In addition, there was a time-dependent accumulation of a high molecular weight complex of ligand and a cellular protein. This complex disappeared when gels were performed under reducing conditions.

Duke Scholars

Published In

J Cell Biochem

DOI

ISSN

0730-2312

Publication Date

1984

Volume

24

Issue

3

Start / End Page

197 / 206

Location

United States

Related Subject Headings

  • Trypsin
  • Thrombin
  • Receptors, Cell Surface
  • Rats, Inbred F344
  • Rats
  • Peptide Hydrolases
  • Male
  • Liver
  • Ligands
  • Kinetics
 

Citation

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Fuchs, H. E., Shifman, M. A., Michalopoulos, G., & Pizzo, S. V. (1984). Hepatocyte receptors for antithrombin III-proteinase complexes. J Cell Biochem, 24(3), 197–206. https://doi.org/10.1002/jcb.240240302
Fuchs, H. E., M. A. Shifman, G. Michalopoulos, and S. V. Pizzo. “Hepatocyte receptors for antithrombin III-proteinase complexes.J Cell Biochem 24, no. 3 (1984): 197–206. https://doi.org/10.1002/jcb.240240302.
Fuchs HE, Shifman MA, Michalopoulos G, Pizzo SV. Hepatocyte receptors for antithrombin III-proteinase complexes. J Cell Biochem. 1984;24(3):197–206.
Fuchs, H. E., et al. “Hepatocyte receptors for antithrombin III-proteinase complexes.J Cell Biochem, vol. 24, no. 3, 1984, pp. 197–206. Pubmed, doi:10.1002/jcb.240240302.
Fuchs HE, Shifman MA, Michalopoulos G, Pizzo SV. Hepatocyte receptors for antithrombin III-proteinase complexes. J Cell Biochem. 1984;24(3):197–206.
Journal cover image

Published In

J Cell Biochem

DOI

ISSN

0730-2312

Publication Date

1984

Volume

24

Issue

3

Start / End Page

197 / 206

Location

United States

Related Subject Headings

  • Trypsin
  • Thrombin
  • Receptors, Cell Surface
  • Rats, Inbred F344
  • Rats
  • Peptide Hydrolases
  • Male
  • Liver
  • Ligands
  • Kinetics