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Defective signaling in a subpopulation of CD4(+) T cells in the absence of Ca(2+)/calmodulin-dependent protein kinase IV.

Publication ,  Journal Article
Anderson, KA; Means, AR
Published in: Mol Cell Biol
January 2002

Ca(2+)/calmodulin-dependent protein kinase IV-deficient (CaMKIV(-/-)) mice have been used to investigate the role of this enzyme in CD4(+) T cells. We identify a functional defect in a subpopulation of CD4(+) T cells, characterized by a cell surface marker profile usually found on memory phenotype CD4(+) T cells. Upon T-cell receptor engagement, the mutant cells produce diminished levels of interleukin-2 (IL-2), IL-4, and gamma interferon protein and mRNA. The defect is secondary to an inability to phosphorylate CREB and to induce CREB-dependent immediate-early genes, including c-jun, fosB, fra2, and junB, which are required for cytokine gene induction. In contrast, stimulated naive CD4(+) T cells from CaMKIV(-/-) mice show normal CREB phosphorylation, induction of immediate-early genes, and cytokine production. Thus, in addition to defining an important signaling role for CaMKIV in a subpopulation of T cells, we identify differential signaling requirements for cytokine production between naive T cells and T cells that express cell surface markers characteristic of the memory phenotype.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

January 2002

Volume

22

Issue

1

Start / End Page

23 / 29

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Signal Transduction
  • Ribosomal Protein S6 Kinases
  • Phenotype
  • Nuclear Proteins
  • NFATC Transcription Factors
  • Mice, Knockout
  • Mice
  • Lymphocyte Activation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Anderson, K. A., & Means, A. R. (2002). Defective signaling in a subpopulation of CD4(+) T cells in the absence of Ca(2+)/calmodulin-dependent protein kinase IV. Mol Cell Biol, 22(1), 23–29. https://doi.org/10.1128/MCB.22.1.23-29.2002

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

January 2002

Volume

22

Issue

1

Start / End Page

23 / 29

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Signal Transduction
  • Ribosomal Protein S6 Kinases
  • Phenotype
  • Nuclear Proteins
  • NFATC Transcription Factors
  • Mice, Knockout
  • Mice
  • Lymphocyte Activation