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p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.

Publication ,  Journal Article
Kerns, BJ; Jordan, PA; Moore, MB; Humphrey, PA; Berchuck, A; Kohler, MF; Bast, RC; Iglehart, JD; Marks, JR
Published in: J Histochem Cytochem
July 1992

Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.

Duke Scholars

Published In

J Histochem Cytochem

DOI

ISSN

0022-1554

Publication Date

July 1992

Volume

40

Issue

7

Start / End Page

1047 / 1051

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tissue Fixation
  • Tissue Embedding
  • Ovarian Neoplasms
  • Male
  • Immunohistochemistry
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Female
  • Carcinoma
 

Citation

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Kerns, B. J., Jordan, P. A., Moore, M. B., Humphrey, P. A., Berchuck, A., Kohler, M. F., … Marks, J. R. (1992). p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry. J Histochem Cytochem, 40(7), 1047–1051. https://doi.org/10.1177/40.7.1607637
Kerns, B. J., P. A. Jordan, M. B. Moore, P. A. Humphrey, A. Berchuck, M. F. Kohler, R. C. Bast, J. D. Iglehart, and J. R. Marks. “p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.J Histochem Cytochem 40, no. 7 (July 1992): 1047–51. https://doi.org/10.1177/40.7.1607637.
Kerns BJ, Jordan PA, Moore MB, Humphrey PA, Berchuck A, Kohler MF, et al. p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry. J Histochem Cytochem. 1992 Jul;40(7):1047–51.
Kerns, B. J., et al. “p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.J Histochem Cytochem, vol. 40, no. 7, July 1992, pp. 1047–51. Pubmed, doi:10.1177/40.7.1607637.
Kerns BJ, Jordan PA, Moore MB, Humphrey PA, Berchuck A, Kohler MF, Bast RC, Iglehart JD, Marks JR. p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry. J Histochem Cytochem. 1992 Jul;40(7):1047–1051.

Published In

J Histochem Cytochem

DOI

ISSN

0022-1554

Publication Date

July 1992

Volume

40

Issue

7

Start / End Page

1047 / 1051

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tissue Fixation
  • Tissue Embedding
  • Ovarian Neoplasms
  • Male
  • Immunohistochemistry
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Female
  • Carcinoma