Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Overexpression and mutation of p53 in endometrial carcinoma.

Publication ,  Journal Article
Kohler, MF; Berchuck, A; Davidoff, AM; Humphrey, PA; Dodge, RK; Iglehart, JD; Soper, JT; Clarke-Pearson, DL; Bast, RC; Marks, JR
Published in: Cancer Res
March 15, 1992

Immunohistochemical staining for the p53 protein was performed in 107 snap frozen primary endometrial adenocarcinomas and 15 benign uterine tissues using monoclonal antibody PAb1801. No staining was seen in benign samples, whereas intense nuclear staining of cancer cells consistent with overexpression of the p53 protein was observed in 22 of 107 cancers (21%). p53 overexpression was more frequent in advanced (Stage III/IV) cancers (41%) than in early (Stage I/II) cancers (9%) (P less than 0.001), and also was associated with nonendometrioid histology (P = 0.008), positive peritoneal cytology (P = 0.01), extrauterine metastases (P = 0.003), and negative progesterone receptor status (P = 0.04). To confirm the relationship between p53 overexpression and mutation, p53 mRNA from 8 cancers was reverse transcribed and amplified using the polymerase chain reaction. DNA sequencing revealed point mutations in each of the 5 cancers that overexpressed p53, whereas the wild-type sequence was found in 3 cancers that did not overexpress the protein. Each of the 5 mutations resulted in an amino acid substitution in a highly conserved region of the p53 gene where mutations have been found in other cancers. Further studies are warranted to determine whether the association between p53 overexpression and advanced stage disease is due to accumulation of genetic lesions during tumor progression or whether p53 alterations confer a more virulent phenotype.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 15, 1992

Volume

52

Issue

6

Start / End Page

1622 / 1627

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Staging
  • Molecular Sequence Data
  • Humans
  • Genes, p53
  • Gene Amplification
  • Female
  • Endometrial Neoplasms
  • Base Sequence
  • Adenocarcinoma
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kohler, M. F., Berchuck, A., Davidoff, A. M., Humphrey, P. A., Dodge, R. K., Iglehart, J. D., … Marks, J. R. (1992). Overexpression and mutation of p53 in endometrial carcinoma. Cancer Res, 52(6), 1622–1627.
Kohler, M. F., A. Berchuck, A. M. Davidoff, P. A. Humphrey, R. K. Dodge, J. D. Iglehart, J. T. Soper, D. L. Clarke-Pearson, R. C. Bast, and J. R. Marks. “Overexpression and mutation of p53 in endometrial carcinoma.Cancer Res 52, no. 6 (March 15, 1992): 1622–27.
Kohler MF, Berchuck A, Davidoff AM, Humphrey PA, Dodge RK, Iglehart JD, et al. Overexpression and mutation of p53 in endometrial carcinoma. Cancer Res. 1992 Mar 15;52(6):1622–7.
Kohler, M. F., et al. “Overexpression and mutation of p53 in endometrial carcinoma.Cancer Res, vol. 52, no. 6, Mar. 1992, pp. 1622–27.
Kohler MF, Berchuck A, Davidoff AM, Humphrey PA, Dodge RK, Iglehart JD, Soper JT, Clarke-Pearson DL, Bast RC, Marks JR. Overexpression and mutation of p53 in endometrial carcinoma. Cancer Res. 1992 Mar 15;52(6):1622–1627.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 15, 1992

Volume

52

Issue

6

Start / End Page

1622 / 1627

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplasm Staging
  • Molecular Sequence Data
  • Humans
  • Genes, p53
  • Gene Amplification
  • Female
  • Endometrial Neoplasms
  • Base Sequence
  • Adenocarcinoma