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Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA.

Publication ,  Journal Article
Nair, SK; Boczkowski, D; Morse, M; Cumming, RI; Lyerly, HK; Gilboa, E
Published in: Nat Biotechnol
April 1998

Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be necessary to generate and/or maintain an optimal CD8+ CTL response in vivo. CEA RNA-transfected DCs also serve as effective targets in cytotoxicity assays, thus providing a general method for inducing, as well as measuring, CEA-specific CTL responses across a broad spectrum of HLA haplotypes.

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Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

April 1998

Volume

16

Issue

4

Start / End Page

364 / 369

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • RNA
  • Neoplasm Metastasis
  • Humans
  • Dendritic Cells
  • Colonic Neoplasms
  • Cell Line
  • Carcinoembryonic Antigen
 

Citation

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Nair, S. K., Boczkowski, D., Morse, M., Cumming, R. I., Lyerly, H. K., & Gilboa, E. (1998). Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA. Nat Biotechnol, 16(4), 364–369. https://doi.org/10.1038/nbt0498-364
Nair, S. K., D. Boczkowski, M. Morse, R. I. Cumming, H. K. Lyerly, and E. Gilboa. “Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA.Nat Biotechnol 16, no. 4 (April 1998): 364–69. https://doi.org/10.1038/nbt0498-364.
Nair SK, Boczkowski D, Morse M, Cumming RI, Lyerly HK, Gilboa E. Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA. Nat Biotechnol. 1998 Apr;16(4):364–9.
Nair, S. K., et al. “Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA.Nat Biotechnol, vol. 16, no. 4, Apr. 1998, pp. 364–69. Pubmed, doi:10.1038/nbt0498-364.
Nair SK, Boczkowski D, Morse M, Cumming RI, Lyerly HK, Gilboa E. Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA. Nat Biotechnol. 1998 Apr;16(4):364–369.

Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

April 1998

Volume

16

Issue

4

Start / End Page

364 / 369

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • RNA
  • Neoplasm Metastasis
  • Humans
  • Dendritic Cells
  • Colonic Neoplasms
  • Cell Line
  • Carcinoembryonic Antigen