Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells.
Publication
, Journal Article
Morse, MA; Lyerly, HK; Gilboa, E; Thomas, E; Nair, SK
Published in: Cancer Res
July 15, 1998
Dendritic cells (DCs), matured by CD40-ligand (CD40L), undergo marked changes in their ability to process and present antigen, resulting in augmented lymphocyte stimulatory activity. We demonstrate that the form of the tumor antigen (peptide or genetic material) used to load the DCs dictates the required sequence of antigen loading and maturation for antitumor immunotherapy. Optimal stimulation of carcinoembryonic antigen (CEA)-specific CTLs by peptide-loaded DCs occurs when DCs from cancer patients are matured with CD40L before exposure to CEA peptide, whereas optimal stimulation by RNA-transfected DCs occurs when the DCs are loaded with CEA RNA before maturation with CD40L.
Duke Scholars
Published In
Cancer Res
ISSN
0008-5472
Publication Date
July 15, 1998
Volume
58
Issue
14
Start / End Page
2965 / 2968
Location
United States
Related Subject Headings
- Transfection
- RNA, Messenger
- Oncology & Carcinogenesis
- Membrane Glycoproteins
- Lymphocyte Activation
- Ligands
- Humans
- Dendritic Cells
- Carcinoembryonic Antigen
- CD40 Ligand
Citation
APA
Chicago
ICMJE
MLA
NLM
Morse, M. A., Lyerly, H. K., Gilboa, E., Thomas, E., & Nair, S. K. (1998). Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells. Cancer Res, 58(14), 2965–2968.
Morse, M. A., H. K. Lyerly, E. Gilboa, E. Thomas, and S. K. Nair. “Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells.” Cancer Res 58, no. 14 (July 15, 1998): 2965–68.
Morse MA, Lyerly HK, Gilboa E, Thomas E, Nair SK. Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells. Cancer Res. 1998 Jul 15;58(14):2965–8.
Morse, M. A., et al. “Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells.” Cancer Res, vol. 58, no. 14, July 1998, pp. 2965–68.
Morse MA, Lyerly HK, Gilboa E, Thomas E, Nair SK. Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells. Cancer Res. 1998 Jul 15;58(14):2965–2968.
Published In
Cancer Res
ISSN
0008-5472
Publication Date
July 15, 1998
Volume
58
Issue
14
Start / End Page
2965 / 2968
Location
United States
Related Subject Headings
- Transfection
- RNA, Messenger
- Oncology & Carcinogenesis
- Membrane Glycoproteins
- Lymphocyte Activation
- Ligands
- Humans
- Dendritic Cells
- Carcinoembryonic Antigen
- CD40 Ligand