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Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone.

Publication ,  Journal Article
Snyder, SA; Lanzen, JL; Braun, RD; Rosner, G; Secomb, TW; Biaglow, J; Brizel, DM; Dewhirst, MW
Published in: Int J Radiat Oncol Biol Phys
October 1, 2001

PURPOSE: To test the feasibility of hyperglycemic reduction of oxygen consumption combined with oxygen breathing (O(2)), to improve tumor oxygenation. METHODS AND MATERIALS: Fischer-344 rats bearing 1 cm R3230Ac flank tumors were anesthetized with Nembutal. Mean arterial pressure, heart rate, tumor blood flow ([TBF], laser Doppler flowmetry), pH, and pO(2) were measured before, during, and after glucose (1 or 4 g/kg) and/or O(2). RESULTS: Mean arterial pressure and heart rate were unaffected by treatment. Glucose at 1 g/kg yielded maximum blood glucose of 400 mg/dL, no change in TBF, reduced tumor pH (0.17 unit), and 3 mm Hg pO(2) rise. Glucose at 4 g/kg yielded maximum blood glucose of 900 mg/dL, pH drop of 0.6 unit, no pO(2) change, and reduced TBF (31%). Oxygen tension increased by 5 mm Hg with O(2). Glucose (1 g/Kg) + O(2) yielded the largest change in pO(2) (27 mm Hg); this is highly significant relative to baseline or either treatment alone. The effect was positively correlated with baseline pO(2), but 6 of 7 experiments with baseline pO(2) < 10 mm Hg rose above 10 mm Hg after combined treatment. CONCLUSION: We demonstrated the feasibility of combining hyperglycemia with O(2) to improve tumor oxygenation. However, some cell lines are not susceptible to the Crabtree effect, and the magnitude is dependent on baseline pO(2). Additional or alternative manipulations may be necessary to achieve more uniform improvement in pO(2).

Duke Scholars

Published In

Int J Radiat Oncol Biol Phys

DOI

ISSN

0360-3016

Publication Date

October 1, 2001

Volume

51

Issue

2

Start / End Page

494 / 506

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Regional Blood Flow
  • Rats, Inbred F344
  • Rats
  • Oxygen Consumption
  • Oxygen
  • Oncology & Carcinogenesis
  • Neoplasms, Experimental
  • Models, Animal
 

Citation

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Snyder, S. A., Lanzen, J. L., Braun, R. D., Rosner, G., Secomb, T. W., Biaglow, J., … Dewhirst, M. W. (2001). Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone. Int J Radiat Oncol Biol Phys, 51(2), 494–506. https://doi.org/10.1016/s0360-3016(01)01654-6
Snyder, S. A., J. L. Lanzen, R. D. Braun, G. Rosner, T. W. Secomb, J. Biaglow, D. M. Brizel, and M. W. Dewhirst. “Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone.Int J Radiat Oncol Biol Phys 51, no. 2 (October 1, 2001): 494–506. https://doi.org/10.1016/s0360-3016(01)01654-6.
Snyder SA, Lanzen JL, Braun RD, Rosner G, Secomb TW, Biaglow J, et al. Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone. Int J Radiat Oncol Biol Phys. 2001 Oct 1;51(2):494–506.
Snyder, S. A., et al. “Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone.Int J Radiat Oncol Biol Phys, vol. 51, no. 2, Oct. 2001, pp. 494–506. Pubmed, doi:10.1016/s0360-3016(01)01654-6.
Snyder SA, Lanzen JL, Braun RD, Rosner G, Secomb TW, Biaglow J, Brizel DM, Dewhirst MW. Simultaneous administration of glucose and hyperoxic gas achieves greater improvement in tumor oxygenation than hyperoxic gas alone. Int J Radiat Oncol Biol Phys. 2001 Oct 1;51(2):494–506.
Journal cover image

Published In

Int J Radiat Oncol Biol Phys

DOI

ISSN

0360-3016

Publication Date

October 1, 2001

Volume

51

Issue

2

Start / End Page

494 / 506

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Regional Blood Flow
  • Rats, Inbred F344
  • Rats
  • Oxygen Consumption
  • Oxygen
  • Oncology & Carcinogenesis
  • Neoplasms, Experimental
  • Models, Animal