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In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.

Publication ,  Journal Article
Chan, BP; Liu, W; Klitzman, B; Reichert, WM; Truskey, GA
Published in: J Biomed Mater Res B Appl Biomater
January 15, 2005

In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of RGD-streptavidin (SA) and biotin to supplement integrin-mediated EC adhesion; (2) cell sodding to pressurize the seeded EC into the interstices of the ePTFE grafts; and (3) longer postseeding attachment time from 1 to 24 h prior to implantation. An in vitro system, which accounts for cell loss due to both graft handling and shear stress, was designed to optimize conditions for in vivo experiments. Results suggest that longer in vitro attachment time enabled the adherent EC to endure mechanical stresses by forming strong adhesions to the underlying extracellular matrix substrates; cell sodding helped to retain the adherent EC by physically docking the cells against the graft interstices; and the SA-biotin interaction enhanced the early attachment of EC but did not lead to better cell retention or reduced surface coverage of blood clot in the current study. Mechanical manipulation of cells during implantation is a limiting factor in maintaining a confluent EC layer on synthetic vascular grafts.

Duke Scholars

Published In

J Biomed Mater Res B Appl Biomater

DOI

ISSN

1552-4973

Publication Date

January 15, 2005

Volume

72

Issue

1

Start / End Page

52 / 63

Location

United States

Related Subject Headings

  • Transplants
  • Thrombosis
  • Polytetrafluoroethylene
  • Microscopy, Electron, Scanning
  • Ligands
  • Humans
  • Femoral Artery
  • Extracellular Matrix
  • Endothelial Cells
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chan, B. P., Liu, W., Klitzman, B., Reichert, W. M., & Truskey, G. A. (2005). In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts. J Biomed Mater Res B Appl Biomater, 72(1), 52–63. https://doi.org/10.1002/jbm.b.30114
Chan, Bernard P., Wenge Liu, Bruce Klitzman, William M. Reichert, and George A. Truskey. “In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.J Biomed Mater Res B Appl Biomater 72, no. 1 (January 15, 2005): 52–63. https://doi.org/10.1002/jbm.b.30114.
Chan BP, Liu W, Klitzman B, Reichert WM, Truskey GA. In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts. J Biomed Mater Res B Appl Biomater. 2005 Jan 15;72(1):52–63.
Chan, Bernard P., et al. “In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.J Biomed Mater Res B Appl Biomater, vol. 72, no. 1, Jan. 2005, pp. 52–63. Pubmed, doi:10.1002/jbm.b.30114.
Chan BP, Liu W, Klitzman B, Reichert WM, Truskey GA. In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts. J Biomed Mater Res B Appl Biomater. 2005 Jan 15;72(1):52–63.
Journal cover image

Published In

J Biomed Mater Res B Appl Biomater

DOI

ISSN

1552-4973

Publication Date

January 15, 2005

Volume

72

Issue

1

Start / End Page

52 / 63

Location

United States

Related Subject Headings

  • Transplants
  • Thrombosis
  • Polytetrafluoroethylene
  • Microscopy, Electron, Scanning
  • Ligands
  • Humans
  • Femoral Artery
  • Extracellular Matrix
  • Endothelial Cells
  • Cells, Cultured