In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts.
In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high-affinity receptor-ligand binding of RGD-streptavidin (SA) and biotin to supplement integrin-mediated EC adhesion; (2) cell sodding to pressurize the seeded EC into the interstices of the ePTFE grafts; and (3) longer postseeding attachment time from 1 to 24 h prior to implantation. An in vitro system, which accounts for cell loss due to both graft handling and shear stress, was designed to optimize conditions for in vivo experiments. Results suggest that longer in vitro attachment time enabled the adherent EC to endure mechanical stresses by forming strong adhesions to the underlying extracellular matrix substrates; cell sodding helped to retain the adherent EC by physically docking the cells against the graft interstices; and the SA-biotin interaction enhanced the early attachment of EC but did not lead to better cell retention or reduced surface coverage of blood clot in the current study. Mechanical manipulation of cells during implantation is a limiting factor in maintaining a confluent EC layer on synthetic vascular grafts.
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- Transplants
- Thrombosis
- Polytetrafluoroethylene
- Microscopy, Electron, Scanning
- Ligands
- Humans
- Femoral Artery
- Extracellular Matrix
- Endothelial Cells
- Cells, Cultured
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transplants
- Thrombosis
- Polytetrafluoroethylene
- Microscopy, Electron, Scanning
- Ligands
- Humans
- Femoral Artery
- Extracellular Matrix
- Endothelial Cells
- Cells, Cultured