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Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling.

Publication ,  Journal Article
Okazaki, MM; McNamara, JO; Nadler, JV
Published in: Neuroscience
1990

The kainate and quisqualate types of excitatory amino acid receptor were visualized autoradiographically in brain sections from rats kindled by stimulating the angular bundle. Kainate receptors were labeled with [3H]kainate and quisqualate receptors with L-[3H]glutamate. When assayed one day after the last evoked seizure, kainate receptor binding had declined by 24-29% in stratum lucidum of hippocampal area CA3 and by 12-14% in the inner third of the dentate molecular layer, but was unchanged in the neocortex and basolateral amygdala. Saturation binding curves revealed that, under the conditions of these experiments, [3H]kainate labeled a single class of binding sites with a KD of 33-36 nM. In stratum lucidum of area CA3, kindling reduced the density of kainate receptors without altering their affinity for kainate. At the same time, quisqualate receptor binding had declined by 20-35% in many layers of the hippocampal formation and neocortex, but remained unchanged in the basolateral amygdala. Repeated stimulation or repeated seizures were required to produce these effects, since both kainate and quisqualate receptor binding were unchanged one day after a single afterdischarge. These receptor changes largely or completely reversed during a 28-day period without further stimulation. Thus maintenance of the kindled state probably cannot be explained by a long-lasting change in the expression of kainate or quisqualate receptors. The transient, regionally-selective down-regulation of these receptors may represent a compensatory response of forebrain neurons to repeated stimulation or seizures.

Duke Scholars

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

1990

Volume

37

Issue

1

Start / End Page

135 / 142

Location

United States

Related Subject Headings

  • Receptors, Neurotransmitter
  • Receptors, Kainic Acid
  • Receptors, AMPA
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Kinetics
  • Kindling, Neurologic
  • Hippocampus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Okazaki, M. M., McNamara, J. O., & Nadler, J. V. (1990). Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling. Neuroscience, 37(1), 135–142. https://doi.org/10.1016/0306-4522(90)90198-d
Okazaki, M. M., J. O. McNamara, and J. V. Nadler. “Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling.Neuroscience 37, no. 1 (1990): 135–42. https://doi.org/10.1016/0306-4522(90)90198-d.
Okazaki MM, McNamara JO, Nadler JV. Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling. Neuroscience. 1990;37(1):135–42.
Okazaki, M. M., et al. “Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling.Neuroscience, vol. 37, no. 1, 1990, pp. 135–42. Pubmed, doi:10.1016/0306-4522(90)90198-d.
Okazaki MM, McNamara JO, Nadler JV. Kainate and quisqualate receptor autoradiography in rat brain after angular bundle kindling. Neuroscience. 1990;37(1):135–142.
Journal cover image

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

1990

Volume

37

Issue

1

Start / End Page

135 / 142

Location

United States

Related Subject Headings

  • Receptors, Neurotransmitter
  • Receptors, Kainic Acid
  • Receptors, AMPA
  • Rats, Inbred Strains
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Kinetics
  • Kindling, Neurologic
  • Hippocampus