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Cloning of murine splenic T lymphocytes and natural killer (NK) cells on filter paper discs: detection of a novel NK/T phenotype.

Publication ,  Journal Article
Sarzotti, M; Kumar, V; Bennett, M; Kelsoe, G
Published in: Eur J Immunol
March 1991

Discrete colonies of splenocytes were grown on filter paper discs in the presence of concanavalin A and interleukin 2. Phenotypic analysis of the colonies indicated that the majority expressed the Thy-1.2 marker and 72% of these co-expressed the CD3 molecule. Of the colonies 20%-25% were NK 1.1+ and they developed regardless of the presence of Con A in the culture medium, a property of the NK lineage. In addition, Thy-1.2+ colonies developed when splenocytes from scid mice, which lack mature T and B cells, were grown both in the presence and absence of concanavalin A. These results demonstrate that colonies of murine splenic T lymphocytes and NK cells could be successfully grown on filter paper discs and phenotypically characterized. With this colonies technique, it was possible to identify a novel subset of NK 1.1+ colonies that co-expresses CD3 and shares growth properties with T cell colonies.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

March 1991

Volume

21

Issue

3

Start / End Page

635 / 641

Location

Germany

Related Subject Headings

  • Thy-1 Antigens
  • T-Lymphocytes
  • Spleen
  • Receptors, Antigen, T-Cell
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Killer Cells, Natural
  • Interleukin-2
  • Immunology
 

Citation

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Sarzotti, M., Kumar, V., Bennett, M., & Kelsoe, G. (1991). Cloning of murine splenic T lymphocytes and natural killer (NK) cells on filter paper discs: detection of a novel NK/T phenotype. Eur J Immunol, 21(3), 635–641. https://doi.org/10.1002/eji.1830210315
Sarzotti, M., V. Kumar, M. Bennett, and G. Kelsoe. “Cloning of murine splenic T lymphocytes and natural killer (NK) cells on filter paper discs: detection of a novel NK/T phenotype.Eur J Immunol 21, no. 3 (March 1991): 635–41. https://doi.org/10.1002/eji.1830210315.
Sarzotti, M., et al. “Cloning of murine splenic T lymphocytes and natural killer (NK) cells on filter paper discs: detection of a novel NK/T phenotype.Eur J Immunol, vol. 21, no. 3, Mar. 1991, pp. 635–41. Pubmed, doi:10.1002/eji.1830210315.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

March 1991

Volume

21

Issue

3

Start / End Page

635 / 641

Location

Germany

Related Subject Headings

  • Thy-1 Antigens
  • T-Lymphocytes
  • Spleen
  • Receptors, Antigen, T-Cell
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Killer Cells, Natural
  • Interleukin-2
  • Immunology