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Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2.

Publication ,  Journal Article
McNulty, AL; Vail, TP; Kraus, VB
Published in: Biochim Biophys Acta
June 30, 2005

Collagen II is the major protein component of articular cartilage and forms the collagen fibril network, which provides the tensile strength of cartilage. Collagen II synthesis is enhanced by ascorbic acid (vitamin C) at both a transcriptional and post-transcriptional level. While the importance of ascorbic acid in the synthesis of collagen has been established, the mechanism by which this essential nutrient is transported into chondrocytes has not been investigated previously. We have characterized the transport of the reduced form of ascorbic acid in passaged primary human chondrocytes to discern the physiologically relevant pathways of ascorbic acid transport in cartilage. We have found that chondrocytes are robust concentrators of ascorbic acid, capable of transporting the reduced form, and concentrating total ascorbic acid, in the reduced form and its metabolites, 960-fold over the concentration in the extracellular milieu. Chondrocyte transport of ascorbic acid was sodium and temperature dependent, stereoselective for the L-forms, and inhibited by the anion transport inhibitor, sulfinpyrazone. Chondrocytes preferentially expressed the full-length and functional isoform of sodium-dependent vitamin C transporter 2 (SVCT2). When this transcript was suppressed with sequence-specific siRNAs, the active transport component of ascorbic acid was abolished. Thus, we provide the first evidence that SVCT2 mediates the secondary active and concentrative transport of ascorbic acid in human chondrocytes.

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Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

June 30, 2005

Volume

1712

Issue

2

Start / End Page

212 / 221

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Temperature
  • Symporters
  • Sulfinpyrazone
  • Sodium-Coupled Vitamin C Transporters
  • Sodium
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA Interference
  • RNA
  • Organic Anion Transporters, Sodium-Dependent
 

Citation

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McNulty, A. L., Vail, T. P., & Kraus, V. B. (2005). Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2. Biochim Biophys Acta, 1712(2), 212–221. https://doi.org/10.1016/j.bbamem.2005.04.009
McNulty, Amy L., Thomas P. Vail, and Virginia B. Kraus. “Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2.Biochim Biophys Acta 1712, no. 2 (June 30, 2005): 212–21. https://doi.org/10.1016/j.bbamem.2005.04.009.
McNulty AL, Vail TP, Kraus VB. Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2. Biochim Biophys Acta. 2005 Jun 30;1712(2):212–21.
McNulty, Amy L., et al. “Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2.Biochim Biophys Acta, vol. 1712, no. 2, June 2005, pp. 212–21. Pubmed, doi:10.1016/j.bbamem.2005.04.009.
McNulty AL, Vail TP, Kraus VB. Chondrocyte transport and concentration of ascorbic acid is mediated by SVCT2. Biochim Biophys Acta. 2005 Jun 30;1712(2):212–221.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

June 30, 2005

Volume

1712

Issue

2

Start / End Page

212 / 221

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Temperature
  • Symporters
  • Sulfinpyrazone
  • Sodium-Coupled Vitamin C Transporters
  • Sodium
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA Interference
  • RNA
  • Organic Anion Transporters, Sodium-Dependent