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Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells.

Publication ,  Journal Article
Kesty, NC; Mason, KM; Reedy, M; Miller, SE; Kuehn, MJ
Published in: EMBO J
November 24, 2004

Enterotoxigenic Escherichia coli (ETEC) is a prevalent cause of traveler's diarrhea and infant mortality in third-world countries. Heat-labile enterotoxin (LT) is secreted from ETEC via vesicles composed of outer membrane and periplasm. We investigated the role of ETEC vesicles in pathogenesis by analyzing vesicle association and entry into eukaryotic cells. Fluorescently labeled vesicles from LT-producing and LT-nonproducing strains were compared in their ability to bind adrenal and intestinal epithelial cells. ETEC-derived vesicles, but not control nonpathogen-derived vesicles, associated with cells in a time-, temperature-, and receptor-dependent manner. Vesicles were visualized on the cell surface at 4 degrees C and detected intracellularly at 37 degrees C. ETEC vesicle endocytosis depended on cholesterol-rich lipid rafts. Entering vesicles partially colocalized with caveolin, and the internalized vesicles accumulated in a nonacidified compartment. We conclude that ETEC vesicles serve as specifically targeted transport vehicles that mediate entry of active enterotoxin and other bacterial envelope components into host cells. These data demonstrate a role in virulence for ETEC vesicles.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

November 24, 2004

Volume

23

Issue

23

Start / End Page

4538 / 4549

Location

England

Related Subject Headings

  • Virulence Factors
  • Virulence
  • Transport Vesicles
  • Periplasm
  • Microscopy, Electron, Transmission
  • Mice
  • Membrane Microdomains
  • Intestinal Mucosa
  • Humans
  • HT29 Cells
 

Citation

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Kesty, N. C., Mason, K. M., Reedy, M., Miller, S. E., & Kuehn, M. J. (2004). Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. EMBO J, 23(23), 4538–4549. https://doi.org/10.1038/sj.emboj.7600471
Kesty, Nicole C., Kevin M. Mason, Mary Reedy, Sara E. Miller, and Meta J. Kuehn. “Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells.EMBO J 23, no. 23 (November 24, 2004): 4538–49. https://doi.org/10.1038/sj.emboj.7600471.
Kesty NC, Mason KM, Reedy M, Miller SE, Kuehn MJ. Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. EMBO J. 2004 Nov 24;23(23):4538–49.
Kesty, Nicole C., et al. “Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells.EMBO J, vol. 23, no. 23, Nov. 2004, pp. 4538–49. Pubmed, doi:10.1038/sj.emboj.7600471.
Kesty NC, Mason KM, Reedy M, Miller SE, Kuehn MJ. Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. EMBO J. 2004 Nov 24;23(23):4538–4549.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

November 24, 2004

Volume

23

Issue

23

Start / End Page

4538 / 4549

Location

England

Related Subject Headings

  • Virulence Factors
  • Virulence
  • Transport Vesicles
  • Periplasm
  • Microscopy, Electron, Transmission
  • Mice
  • Membrane Microdomains
  • Intestinal Mucosa
  • Humans
  • HT29 Cells