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Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides.

Publication ,  Journal Article
Berwin, B; Hart, JP; Pizzo, SV; Nicchitta, CV
Published in: J Immunol
May 1, 2002

GRP94(gp96) elicits CD8(+) T cell responses against its bound peptides, a process requiring access of its associated peptides into the MHC class I cross-presentation pathway of APCs. Entry into this pathway requires receptor-mediated endocytosis, and CD91 (low-density lipoprotein receptor-related protein) has been reported to be the receptor mediating GRP94 uptake into APC. However, a direct role for CD91 in chaperone-based peptide Ag re-presentation has not been demonstrated. We investigated the contribution of CD91 to GRP94 cell surface binding, internalization, and trafficking in APCs. Whereas a clear role for CD91 in alpha(2)-macroglobulin binding and uptake was readily obtained, the addition of excess CD91 ligand, activated alpha(2)-macroglobulin, or receptor-associated protein, an antagonist of all known CD91 ligands, did not affect GRP94 cell surface binding, receptor-mediated endocytosis, or peptide re-presentation. These data identify a CD91-independent, GRP94 internalization pathway that functions in peptide Ag re-presentation.

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Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

May 1, 2002

Volume

168

Issue

9

Start / End Page

4282 / 4286

Location

England

Related Subject Headings

  • alpha-Macroglobulins
  • Protein Transport
  • Peptides
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • LDL-Receptor Related Protein-Associated Protein
  • Immunology
  • Endocytosis
 

Citation

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Berwin, B., Hart, J. P., Pizzo, S. V., & Nicchitta, C. V. (2002). Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides. J Immunol, 168(9), 4282–4286. https://doi.org/10.4049/jimmunol.168.9.4282
Berwin, Brent, Justin P. Hart, Salvatore V. Pizzo, and Christopher V. Nicchitta. “Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides.J Immunol 168, no. 9 (May 1, 2002): 4282–86. https://doi.org/10.4049/jimmunol.168.9.4282.
Berwin B, Hart JP, Pizzo SV, Nicchitta CV. Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides. J Immunol. 2002 May 1;168(9):4282–6.
Berwin, Brent, et al. “Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides.J Immunol, vol. 168, no. 9, May 2002, pp. 4282–86. Pubmed, doi:10.4049/jimmunol.168.9.4282.
Berwin B, Hart JP, Pizzo SV, Nicchitta CV. Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides. J Immunol. 2002 May 1;168(9):4282–4286.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

May 1, 2002

Volume

168

Issue

9

Start / End Page

4282 / 4286

Location

England

Related Subject Headings

  • alpha-Macroglobulins
  • Protein Transport
  • Peptides
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • LDL-Receptor Related Protein-Associated Protein
  • Immunology
  • Endocytosis