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Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.

Publication ,  Journal Article
Pedrotty, DM; Klinger, RY; Badie, N; Hinds, S; Kardashian, A; Bursac, N
Published in: Am J Physiol Heart Circ Physiol
July 2008

Well-controlled studies of the structural and functional interactions between cardiomyocytes and other cells are essential for understanding heart pathophysiology and for the further development of safe and efficient cell therapies. We established a novel in vitro assay composed of a large number of individual micropatterned cell pairs with reproducible shape, size, and region of cell-cell contact. This assay was applied to quantify and compare the frequency of expression and distribution of electrical (connexin43) and mechanical (N-cadherin) coupling proteins in 5,000 cell pairs made of cardiomyocytes (CMs), cardiac fibroblasts (CFs), skeletal myoblasts (SKMs), and mesenchymal stem cells (MSCs). We found that for all cell pair types, side-side contacts between two cells formed 4.5-14.3 times more often than end-end contacts. Both connexin43 and N-cadherin were expressed in all homotypic CM pairs but in only 13.4-91.6% of pairs containing noncardiomyocytes, where expression was either junctional (at the site of cell-cell contact) or diffuse (inside the cytoplasm). CM expression was exclusively junctional in homotypic pairs but predominantly diffuse in heterotypic pairs. Noncardiomyocyte homotypic pairs exhibited diffuse expression 1.7-8.7 times more often than junctional expression, which was increased 2.6-4.4 times in heterotypic pairs. Junctional connexin43 and N-cadherin expression, respectively, were found in 38.6 +/- 7.3 and 39.6 +/- 6.2% of CM-MSC pairs, 21.9 +/- 5.0 and 13.6 +/- 1.9% of CM-SKM pairs, and in only 3.8-9.6% of CM-CF pairs. Measured frequencies of protein expression and distribution were stable for at least 4 days. Described studies in micropatterned cell pairs shed new light on cellular interactions relevant for cardiac function and cell therapies.

Duke Scholars

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

July 2008

Volume

295

Issue

1

Start / End Page

H390 / H400

Location

United States

Related Subject Headings

  • Time Factors
  • Reproducibility of Results
  • Rats, Sprague-Dawley
  • Rats
  • Myocytes, Cardiac
  • Myoblasts, Skeletal
  • Mesenchymal Stem Cells
  • Intercellular Junctions
  • Fibroblasts
  • Cytoplasm
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pedrotty, D. M., Klinger, R. Y., Badie, N., Hinds, S., Kardashian, A., & Bursac, N. (2008). Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay. Am J Physiol Heart Circ Physiol, 295(1), H390–H400. https://doi.org/10.1152/ajpheart.91531.2007
Pedrotty, Dawn M., Rebecca Y. Klinger, Nima Badie, Sara Hinds, Ara Kardashian, and Nenad Bursac. “Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.Am J Physiol Heart Circ Physiol 295, no. 1 (July 2008): H390–400. https://doi.org/10.1152/ajpheart.91531.2007.
Pedrotty DM, Klinger RY, Badie N, Hinds S, Kardashian A, Bursac N. Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay. Am J Physiol Heart Circ Physiol. 2008 Jul;295(1):H390–400.
Pedrotty, Dawn M., et al. “Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.Am J Physiol Heart Circ Physiol, vol. 295, no. 1, July 2008, pp. H390–400. Pubmed, doi:10.1152/ajpheart.91531.2007.
Pedrotty DM, Klinger RY, Badie N, Hinds S, Kardashian A, Bursac N. Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay. Am J Physiol Heart Circ Physiol. 2008 Jul;295(1):H390–H400.

Published In

Am J Physiol Heart Circ Physiol

DOI

ISSN

0363-6135

Publication Date

July 2008

Volume

295

Issue

1

Start / End Page

H390 / H400

Location

United States

Related Subject Headings

  • Time Factors
  • Reproducibility of Results
  • Rats, Sprague-Dawley
  • Rats
  • Myocytes, Cardiac
  • Myoblasts, Skeletal
  • Mesenchymal Stem Cells
  • Intercellular Junctions
  • Fibroblasts
  • Cytoplasm