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Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.

Publication ,  Journal Article
Pyhtila, JW; Ma, H; Simnick, AJ; Chilkoti, A; Wax, A
Published in: Journal of biomedical optics
May 2006

Angle-resolved low coherence interferometry (a/LCI) enables depth-resolved measurements of scattered light that can be used to recover subsurface structural information, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be complicated by coherent scattering between adjacent cell nuclei. Previous studies have eliminated this component by applying a window filter to Fourier transformed angular data, based on the justification that the coherent scattering must necessarily occur over length scales greater than the cell size. To fully study this effect, results of experiments designed to test the validity of this approach are now presented. The a/LCI technique is used to examine light scattered by regular cell arrays, created using stamped adhesive micropatterned substrates. By varying the array spacing, it is demonstrated that cell-to-cell correlations have a predictable effect on light scattering distributions. These results are compared to image analysis of fluorescence micrographs of the cell array samples. The a/LCI results show that the impact of coherent scattering on nuclear morphology measurements can be eliminated through data filtering.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

May 2006

Volume

11

Issue

3

Start / End Page

34022

Related Subject Headings

  • Tomography, Optical Coherence
  • Statistics as Topic
  • Sensitivity and Specificity
  • Scattering, Radiation
  • Reproducibility of Results
  • Optics
  • NIH 3T3 Cells
  • Mice
  • Light
  • Interferometry
 

Citation

APA
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ICMJE
MLA
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Pyhtila, J. W., Ma, H., Simnick, A. J., Chilkoti, A., & Wax, A. (2006). Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry. Journal of Biomedical Optics, 11(3), 34022. https://doi.org/10.1117/1.2209561
Pyhtila, John W., Hongwei Ma, Andrew J. Simnick, Ashutosh Chilkoti, and Adam Wax. “Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.Journal of Biomedical Optics 11, no. 3 (May 2006): 34022. https://doi.org/10.1117/1.2209561.
Pyhtila JW, Ma H, Simnick AJ, Chilkoti A, Wax A. Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry. Journal of biomedical optics. 2006 May;11(3):34022.
Pyhtila, John W., et al. “Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry.Journal of Biomedical Optics, vol. 11, no. 3, May 2006, p. 34022. Epmc, doi:10.1117/1.2209561.
Pyhtila JW, Ma H, Simnick AJ, Chilkoti A, Wax A. Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry. Journal of biomedical optics. 2006 May;11(3):34022.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

May 2006

Volume

11

Issue

3

Start / End Page

34022

Related Subject Headings

  • Tomography, Optical Coherence
  • Statistics as Topic
  • Sensitivity and Specificity
  • Scattering, Radiation
  • Reproducibility of Results
  • Optics
  • NIH 3T3 Cells
  • Mice
  • Light
  • Interferometry