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Label-free plasmonic detection of biomolecular binding by a single gold nanorod.

Publication ,  Journal Article
Nusz, GJ; Marinakos, SM; Curry, AC; Dahlin, A; Höök, F; Wax, A; Chilkoti, A
Published in: Analytical chemistry
February 2008

We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system. The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold lower than the previously reported detection limit for streptavidin binding by biotinylated single plasmonic nanostructures. We believe that the current optical setup is able to reliably measure wavelength shifts as small as 0.3 nm. Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 0.59 nm suggesting that we are currently operating at close to the limit of detection of the system.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2008

Volume

80

Issue

4

Start / End Page

984 / 989

Related Subject Headings

  • Time Factors
  • Surface Properties
  • Surface Plasmon Resonance
  • Streptavidin
  • Sensitivity and Specificity
  • Particle Size
  • Nanotubes
  • Microscopy, Atomic Force
  • Metal Nanoparticles
  • Gold
 

Citation

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Nusz, G. J., Marinakos, S. M., Curry, A. C., Dahlin, A., Höök, F., Wax, A., & Chilkoti, A. (2008). Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Analytical Chemistry, 80(4), 984–989. https://doi.org/10.1021/ac7017348
Nusz, Greg J., Stella M. Marinakos, Adam C. Curry, Andreas Dahlin, Fredrik Höök, Adam Wax, and Ashutosh Chilkoti. “Label-free plasmonic detection of biomolecular binding by a single gold nanorod.Analytical Chemistry 80, no. 4 (February 2008): 984–89. https://doi.org/10.1021/ac7017348.
Nusz GJ, Marinakos SM, Curry AC, Dahlin A, Höök F, Wax A, et al. Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Analytical chemistry. 2008 Feb;80(4):984–9.
Nusz, Greg J., et al. “Label-free plasmonic detection of biomolecular binding by a single gold nanorod.Analytical Chemistry, vol. 80, no. 4, Feb. 2008, pp. 984–89. Epmc, doi:10.1021/ac7017348.
Nusz GJ, Marinakos SM, Curry AC, Dahlin A, Höök F, Wax A, Chilkoti A. Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Analytical chemistry. 2008 Feb;80(4):984–989.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2008

Volume

80

Issue

4

Start / End Page

984 / 989

Related Subject Headings

  • Time Factors
  • Surface Properties
  • Surface Plasmon Resonance
  • Streptavidin
  • Sensitivity and Specificity
  • Particle Size
  • Nanotubes
  • Microscopy, Atomic Force
  • Metal Nanoparticles
  • Gold