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Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo.

Publication ,  Journal Article
Palmer, GM; Viola, RJ; Schroeder, T; Yarmolenko, PS; Dewhirst, MW; Ramanujam, N
Published in: J Biomed Opt
2009

This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% O(2), 5% CO(2)). In particular, significant increases in hemoglobin saturation and fluorescence redox ratio were observed upon carbogen breathing. These data were compared with data obtained concurrently using an established invasive technique, the OxyLite partial oxygen pressure (pO(2)) system, which also showed a significant increase in pO(2). It was found that the direction of changes were generally the same between all of the methods, but that the OxyLite system was much more variable in general, suggesting that optical techniques may provide a better assessment of global tumor physiology. Optical spectroscopy measurements are demonstrated to provide a reliable, reproducible indication of changes in tumor physiology in response to physiologic manipulation.

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Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2009

Volume

14

Issue

2

Start / End Page

024010

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Photometry
  • Oxygen Consumption
  • Oxygen
  • Optics
  • Mice
  • Mammary Neoplasms, Experimental
  • Animals
  • Algorithms
  • 5102 Atomic, molecular and optical physics
 

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Palmer, G. M., Viola, R. J., Schroeder, T., Yarmolenko, P. S., Dewhirst, M. W., & Ramanujam, N. (2009). Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo. J Biomed Opt, 14(2), 024010. https://doi.org/10.1117/1.3103586
Palmer, Gregory M., Ronald J. Viola, Thies Schroeder, Pavel S. Yarmolenko, Mark W. Dewhirst, and Nirmala Ramanujam. “Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo.J Biomed Opt 14, no. 2 (2009): 024010. https://doi.org/10.1117/1.3103586.
Palmer GM, Viola RJ, Schroeder T, Yarmolenko PS, Dewhirst MW, Ramanujam N. Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo. J Biomed Opt. 2009;14(2):024010.
Palmer, Gregory M., et al. “Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo.J Biomed Opt, vol. 14, no. 2, 2009, p. 024010. Pubmed, doi:10.1117/1.3103586.
Palmer GM, Viola RJ, Schroeder T, Yarmolenko PS, Dewhirst MW, Ramanujam N. Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo. J Biomed Opt. 2009;14(2):024010.

Published In

J Biomed Opt

DOI

ISSN

1083-3668

Publication Date

2009

Volume

14

Issue

2

Start / End Page

024010

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Photometry
  • Oxygen Consumption
  • Oxygen
  • Optics
  • Mice
  • Mammary Neoplasms, Experimental
  • Animals
  • Algorithms
  • 5102 Atomic, molecular and optical physics