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Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine.

Publication ,  Journal Article
Beagley, KW; Fujihashi, K; Lagoo, AS; Lagoo-Deenadaylan, S; Black, CA; Murray, AM; Sharmanov, AT; Yamamoto, M; McGhee, JR; Elson, CO
Published in: J Immunol
June 1, 1995

Intraepithelial lymphocytes (IELs) have been extensively studied in the murine small intestine. However, to date no studies have assessed IEL in the large intestine, despite the marked differences in function and lumenal environment. In the present study, we isolated IEL from both small and large intestine of three mouse strains (BALB/c, C3H/HeN, C57BL/6) and determined the frequency of CD2, CD4, and CD8 expression on CD3+ IEL, as well as the frequency of alpha beta and gamma delta TCR usage and V beta distribution. Higher numbers of IEL/unit length were always isolated from the small intestine (20-30 x 10(6)/5 mice) compared with large intestine (1.1-2.5 x 10(6)/5 mice). Interestingly, IEL from the large intestine of all strains were predominantly alpha beta TCR+ whereas gamma delta TCR+ IELs predominated in small intestine. Large intestinal IELs were mainly CD4+, in both BALB/c and C3H/HeN mouse strains. IELs from large intestine of C57BL/6 mice were mainly CD8+; however, the CD4+ subset was fourfold higher when compared with small intestine IEL. Potential functional differences between IEL subsets was assessed by determining the relative levels of mRNA for IL-1, 2, 4, 5, 10, IFN-gamma, TGF-beta, and TNF-gamma. Similar patterns of IL-1, IFN-gamma and TNF-alpha were seen while more IL-2, IL-4, IL-5, and IL-10 mRNA was noted in large intestinal IEL. Stimulation of C3H/HeJ IEL with anti-CD3 also resulted in higher levels of IL-3/GM-CSF, IL-4, and IL-6 by IEL from large intestine. These results show that marked differences occur among the T cell subsets present in IELs from mouse small and large intestine.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1, 1995

Volume

154

Issue

11

Start / End Page

5611 / 5619

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Mice, Inbred C3H
  • Mice
  • Lymphocyte Activation
  • Intestine, Small
  • Intestine, Large
  • Intestinal Mucosa
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Beagley, K. W., Fujihashi, K., Lagoo, A. S., Lagoo-Deenadaylan, S., Black, C. A., Murray, A. M., … Elson, C. O. (1995). Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine. J Immunol, 154(11), 5611–5619.
Beagley, K. W., K. Fujihashi, A. S. Lagoo, S. Lagoo-Deenadaylan, C. A. Black, A. M. Murray, A. T. Sharmanov, M. Yamamoto, J. R. McGhee, and C. O. Elson. “Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine.J Immunol 154, no. 11 (June 1, 1995): 5611–19.
Beagley KW, Fujihashi K, Lagoo AS, Lagoo-Deenadaylan S, Black CA, Murray AM, et al. Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine. J Immunol. 1995 Jun 1;154(11):5611–9.
Beagley, K. W., et al. “Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine.J Immunol, vol. 154, no. 11, June 1995, pp. 5611–19.
Beagley KW, Fujihashi K, Lagoo AS, Lagoo-Deenadaylan S, Black CA, Murray AM, Sharmanov AT, Yamamoto M, McGhee JR, Elson CO. Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine. J Immunol. 1995 Jun 1;154(11):5611–5619.

Published In

J Immunol

ISSN

0022-1767

Publication Date

June 1, 1995

Volume

154

Issue

11

Start / End Page

5611 / 5619

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Mice, Inbred C3H
  • Mice
  • Lymphocyte Activation
  • Intestine, Small
  • Intestine, Large
  • Intestinal Mucosa
  • Immunology