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Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment.

Publication ,  Journal Article
Lagoo, AS; Gong, JZ; Stenzel, TT; Goodman, BK; Buckley, PJ; Chao, NJ; Gasparetto, C; Long, GD; Rizzieri, DA
Published in: Arch Pathol Lab Med
October 2006

CONTEXT: Nonmyeloablative stem cell transplantation (NMSCT) is a mode of immunotherapy increasingly employed in treating hematologic, lymphoid, and solid tumors. Patients are monitored principally by molecular analysis of donor engraftment. OBJECTIVE: To determine the role of morphologic examination of bone marrow after NMSCT. DESIGN: Seventy-three patients undergoing NMSCT under the Campath 1H (humanized anti-CD52 antibody) protocol were studied. Pretransplant and sequential posttransplant bone marrow specimens were evaluated and the findings were correlated with corresponding engraftment data. RESULTS: Pretransplant bone marrow specimens from 43% of the patients were involved by disease, and these marrow specimens were significantly more cellular than those that were free of disease. Morphologically detectable disease was still present in day 14 posttransplant marrow specimens in more than one half of these patients, but there was no difference in engraftment in those with or without marrow disease. Early posttransplant marrow in nearly one half of the patients showed myeloid hyperplasia and atypical localization of immature myeloid precursors. Marrow cellularity for the first 2 months after NMSCT was significantly lower in those patients receiving stem cells mismatched at 1 to 3 loci as compared with those who received fully matched grafts (mean cellularity, 38.1% vs 54.1% at day 14). Marrow failure without recurrent disease at 3 to 6 months after transplant was detected by engraftment study in only approximately 15% of cases. Similarly, early recurrence of disease was detected first by morphologic examination in 4 of 13 cases before a decline in donor engraftment occurred. CONCLUSION: Morphologic examination of bone marrow provides additional information that is complementary to donor engraftment analysis for optimal management after NMSCT.

Duke Scholars

Published In

Arch Pathol Lab Med

DOI

EISSN

1543-2165

Publication Date

October 2006

Volume

130

Issue

10

Start / End Page

1479 / 1488

Location

United States

Related Subject Headings

  • Tissue Donors
  • Time Factors
  • Stem Cell Transplantation
  • Preoperative Care
  • Postoperative Period
  • Pathology
  • Neoplasms
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Lymphoproliferative Disorders
 

Citation

APA
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ICMJE
MLA
NLM
Lagoo, A. S., Gong, J. Z., Stenzel, T. T., Goodman, B. K., Buckley, P. J., Chao, N. J., … Rizzieri, D. A. (2006). Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Arch Pathol Lab Med, 130(10), 1479–1488. https://doi.org/10.5858/2006-130-1479-MEOSBM
Lagoo, Anand S., Jerald Z. Gong, Timothy T. Stenzel, Barbara K. Goodman, Patrick J. Buckley, Nelson J. Chao, Cristina Gasparetto, Gwynn D. Long, and David A. Rizzieri. “Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment.Arch Pathol Lab Med 130, no. 10 (October 2006): 1479–88. https://doi.org/10.5858/2006-130-1479-MEOSBM.
Lagoo AS, Gong JZ, Stenzel TT, Goodman BK, Buckley PJ, Chao NJ, et al. Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Arch Pathol Lab Med. 2006 Oct;130(10):1479–88.
Lagoo, Anand S., et al. “Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment.Arch Pathol Lab Med, vol. 130, no. 10, Oct. 2006, pp. 1479–88. Pubmed, doi:10.5858/2006-130-1479-MEOSBM.
Lagoo AS, Gong JZ, Stenzel TT, Goodman BK, Buckley PJ, Chao NJ, Gasparetto C, Long GD, Rizzieri DA. Morphologic examination of sequential bone marrow biopsies after nonmyeloablative stem cell transplantation complements molecular studies of donor engraftment. Arch Pathol Lab Med. 2006 Oct;130(10):1479–1488.

Published In

Arch Pathol Lab Med

DOI

EISSN

1543-2165

Publication Date

October 2006

Volume

130

Issue

10

Start / End Page

1479 / 1488

Location

United States

Related Subject Headings

  • Tissue Donors
  • Time Factors
  • Stem Cell Transplantation
  • Preoperative Care
  • Postoperative Period
  • Pathology
  • Neoplasms
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Lymphoproliferative Disorders