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IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp).

Publication ,  Journal Article
Ferrari, G; King, K; Rathbun, K; Place, CA; Packard, MV; Bartlett, JA; Bolognesi, DP; Weinhold, KJ
Published in: Clin Exp Immunol
August 1995

CD8+ cytotoxic T lymphocytes are an important component in the immunologic control of human viral diseases. IL-7, a stromal cell-derived cytokine, has been demonstrated to enhance both anti-tumour and anti-viral CTL as well as lymphokine-activated killer (LAK) activity. We studied the ability of IL-7 to support the activation and the growth of in vitro antigen-specific CTL precursors (CTLp) present in peripheral blood mononuclear cells (PBMC) from HIV-infected patients. Results from these studies demonstrate that inclusion of IL-7 in a vaccinia/HIV-1 vector-based stimulation strategy greatly augmented overall CTL reactivities, whereas addition of IL-7 to unstimulated cultures failed to induce any significant anti-viral cytolytic activity. In four of six patients, HIV-specific lytic activities were significantly higher in cultures stimulated with antigen plus IL-7 compared with in vitro stimulation (IVS) with antigen alone. Cytotoxic activity was principally mediated by CD8+ effector cells, and CD3+/CD8+ cell expansion was increased by 2.7-fold in the presence of IL-7. In PBMC from seronegative donors, IL-7 enhanced anti-vaccinia CTL activities with less effect on cell proliferation. Furthermore, anti-gag CTL frequencies determined by limiting dilution analysis were increased by 2- and 10-fold in two asymptomatic patients following IVS plus IL-7 compared with antigen stimulation alone. Cytofluorimetric analysis revealed that IL-7 preferentially expanded CD8 memory cells (CD45RO+) and CD8+ lymphocytes expressing activation molecules. IL-7 was also able to support the growth of CD4+ lymphocytes, while having no effect on natural killer (NK)/K lymphocytes. Taken together, these data suggest that IL-7 acts cooperatively with the antigen supporting in vitro maturation of CTLp into functional cytotoxic effectors. Thus IL-7 may be an important biologic entity to consider as part of future immune-based therapies in which ex vivo expansion of antigen-driven CTL is an important determinant.

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Published In

Clin Exp Immunol

DOI

ISSN

0009-9104

Publication Date

August 1995

Volume

101

Issue

2

Start / End Page

239 / 248

Location

England

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • T-Lymphocyte Subsets
  • Recombinant Proteins
  • Mice, Inbred C3H
  • Mice
  • Lymphocyte Activation
  • Interleukin-7
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
 

Citation

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MLA
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Ferrari, G., King, K., Rathbun, K., Place, C. A., Packard, M. V., Bartlett, J. A., … Weinhold, K. J. (1995). IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp). Clin Exp Immunol, 101(2), 239–248. https://doi.org/10.1111/j.1365-2249.1995.tb08345.x
Ferrari, G., K. King, K. Rathbun, C. A. Place, M. V. Packard, J. A. Bartlett, D. P. Bolognesi, and K. J. Weinhold. “IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp).Clin Exp Immunol 101, no. 2 (August 1995): 239–48. https://doi.org/10.1111/j.1365-2249.1995.tb08345.x.
Ferrari G, King K, Rathbun K, Place CA, Packard MV, Bartlett JA, et al. IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp). Clin Exp Immunol. 1995 Aug;101(2):239–48.
Ferrari, G., et al. “IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp).Clin Exp Immunol, vol. 101, no. 2, Aug. 1995, pp. 239–48. Pubmed, doi:10.1111/j.1365-2249.1995.tb08345.x.
Ferrari G, King K, Rathbun K, Place CA, Packard MV, Bartlett JA, Bolognesi DP, Weinhold KJ. IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp). Clin Exp Immunol. 1995 Aug;101(2):239–248.
Journal cover image

Published In

Clin Exp Immunol

DOI

ISSN

0009-9104

Publication Date

August 1995

Volume

101

Issue

2

Start / End Page

239 / 248

Location

England

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • T-Lymphocyte Subsets
  • Recombinant Proteins
  • Mice, Inbred C3H
  • Mice
  • Lymphocyte Activation
  • Interleukin-7
  • Immunology
  • Humans
  • Hematopoietic Stem Cells