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Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis.

Publication ,  Journal Article
Stryjewski, ME; Hall, RP; Chu, VH; Kanafani, ZA; O'Riordan, WD; Weinstock, MS; Stienecker, RS; Streilein, R; Dorschner, RA; Fowler, VG ...
Published in: J Infect Dis
November 1, 2007

BACKGROUND: Endogenous antimicrobial peptides participate in the innate defense of skin against a variety of pathogens. The systemic expression of these peptides in normal-appearing skin of patients with infective cellulitis is unknown. METHODS: Study patients were adults with infective cellulitis and signs of systemic inflammation. Skin biopsy and serum specimens were obtained from patients and from control subjects with no active infection. Cathelicidin and human beta-defensin 2 mRNA expression were determined by real-time polymerase chain reaction. RESULTS: Skin biopsy specimens from 11 patients and 4 uninfected control subjects were analyzed. The relative expression level for cathelicidin mRNA was elevated in both the involved and the distal normal-appearing skin of patients with cellulitis, compared with expression in the skin of control subjects (mean ratios, 39.46 vs. 1.32, P=.0059; and 21.41 vs. 1.32, P=.0059). Similarly, the relative expression level of human beta -defensin 2 mRNA was elevated in both the involved skin (mean ratios, 20,844 vs. 11.65; P=.0015) and in distal normal-appearing skin of patients with cellulitis (mean ratios, 201.1 vs. 11.65; P=.0103). DISCUSSION: In response to cutaneous infection there is a local and distal increase in endogenous antimicrobial peptide mRNA in both involved and normal-appearing skin. These observations show, for the first time to our knowledge, that after infection the human body responds by increasing systemic innate immunity.

Duke Scholars

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

November 1, 2007

Volume

196

Issue

9

Start / End Page

1425 / 1430

Location

United States

Related Subject Headings

  • beta-Defensins
  • Skin
  • RNA, Messenger
  • Middle Aged
  • Microbiology
  • Male
  • Interleukin-6
  • Humans
  • Gene Expression Regulation
  • Female
 

Citation

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Stryjewski, M. E., Hall, R. P., Chu, V. H., Kanafani, Z. A., O’Riordan, W. D., Weinstock, M. S., … Gallo, R. L. (2007). Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis. J Infect Dis, 196(9), 1425–1430. https://doi.org/10.1086/522630
Stryjewski, Martin E., Russell P. Hall, Vivian H. Chu, Zeina A. Kanafani, William D. O’Riordan, Michael S. Weinstock, R Scott Stienecker, et al. “Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis.J Infect Dis 196, no. 9 (November 1, 2007): 1425–30. https://doi.org/10.1086/522630.
Stryjewski ME, Hall RP, Chu VH, Kanafani ZA, O’Riordan WD, Weinstock MS, et al. Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis. J Infect Dis. 2007 Nov 1;196(9):1425–30.
Stryjewski, Martin E., et al. “Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis.J Infect Dis, vol. 196, no. 9, Nov. 2007, pp. 1425–30. Pubmed, doi:10.1086/522630.
Stryjewski ME, Hall RP, Chu VH, Kanafani ZA, O’Riordan WD, Weinstock MS, Stienecker RS, Streilein R, Dorschner RA, Fowler VG, Corey GR, Gallo RL. Expression of antimicrobial peptides in the normal and involved skin of patients with infective cellulitis. J Infect Dis. 2007 Nov 1;196(9):1425–1430.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

November 1, 2007

Volume

196

Issue

9

Start / End Page

1425 / 1430

Location

United States

Related Subject Headings

  • beta-Defensins
  • Skin
  • RNA, Messenger
  • Middle Aged
  • Microbiology
  • Male
  • Interleukin-6
  • Humans
  • Gene Expression Regulation
  • Female