Scholars@Duke publication: Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange.

Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange.

Publication , Journal Article

Bond, JE; Cianciolo, GJ; Pizzo, SV

Published in: Biochem Biophys Res Commun

June 1, 2007

alpha(2)-Macroglobulin (alpha(2)M) is a proteinase inhibitor that functions by a trapping mechanism which has been exploited such that the receptor-recognized, activated form (alpha(2)M( *)) can be employed to target antigens to antigen-presenting cells. Another potential use of alpha(2)M( *) is as a drug delivery system. In this study we demonstrate that guanosine triphosphate, labeled with Texas red (GTP-TR) formed complexes with alpha(2)M( *) following activation by proteolytic or non-proteolytic reactions. Optimal incorporation occurred with 20 microM GTP-TR, pH 8.0 for 5h at 50 degrees C. NaCl concentration (100 or 200 mM) had little effect on incorporation at this pH or temperature, but was significant at sub-optimum temperature and pH values. Maximum incorporation was 1.2 mol GTP-TR/mol alpha(2)M( *). PAGE showed that 70-90% of the GTP-TR is bound in a SDS/2-mercaptoethanol resistant manner. Guanosine, adenosine, and imidazole competed with GTP-TR to form complexes with alpha(2)M( *).

Duke Scholars

Author George James Cianciolo Pathology

Author Salvatore Vincent Pizzo Pathology

Published In

Biochem Biophys Res Commun

DOI

10.1016/j.bbrc.2007.03.151

ISSN

0006-291X

Publication Date

June 1, 2007

Volume

357

Issue

Start / End Page

433 / 438

Location

United States

Related Subject Headings

alpha-Macroglobulins
Staining and Labeling
Protein Engineering
Protein Binding
Nucleosides
Molecular Weight
Biochemistry & Molecular Biology
Binding Sites
3404 Medicinal and biomolecular chemistry
3101 Biochemistry and cell biology

Citation

APA

Chicago

ICMJE

MLA

NLM

Bond, J. E., Cianciolo, G. J., & Pizzo, S. V. (2007). Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange. Biochem Biophys Res Commun, 357(2), 433–438. https://doi.org/10.1016/j.bbrc.2007.03.151

Bond, Jennifer E., George J. Cianciolo, and Salvatore V. Pizzo. “Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange.” Biochem Biophys Res Commun 357, no. 2 (June 1, 2007): 433–38. https://doi.org/10.1016/j.bbrc.2007.03.151.

Bond JE, Cianciolo GJ, Pizzo SV. Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange. Biochem Biophys Res Commun. 2007 Jun 1;357(2):433–8.

Bond, Jennifer E., et al. “Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange.” Biochem Biophys Res Commun, vol. 357, no. 2, June 2007, pp. 433–38. Pubmed, doi:10.1016/j.bbrc.2007.03.151.

Bond JE, Cianciolo GJ, Pizzo SV. Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange. Biochem Biophys Res Commun. 2007 Jun 1;357(2):433–438.