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Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity.

Publication ,  Journal Article
Pisetsky, DS; Hoch, SO; Klatt, CL; O'Donnell, MA; Keene, JD
Published in: J Immunol
December 1985

To investigate the mechanisms of anti-Sm expression in murine systemic lupus erythematosus (SLE), the idiotypic determinants of a monoclonal anti-Sm antibody were studied. This antibody, 2G7, was derived from the fusion of spleen cells of an autoimmune MRL-lpr/lpr mouse with the 653 myeloma. Specificity for Sm was demonstrated by an ELISA with the use of affinity-purified Sm as well as immunoprecipitation of radiolabeled RNA. An anti-2G7 antiserum was prepared in a rabbit and rendered specific for idiotype by extensive absorption against BALB/c and MRL-lpr/lpr monoclonal proteins. The resulting antiserum detected determinants found on 2G7 as well as on two MRL anti-DNA antibodies, 6/P and 6/N, an independently derived MRL-lpr/lpr anti-Sm called 7.13, and the BALB/c myeloma FLOPC 21. Two distinct determinants could be demonstrated by the creation of cross-reactive idiotype systems by using the various monoclonal antibodies as ligands for the anti-idiotype. Both idiotypes were demonstrated in sera of normal and autoimmune mice, although MRL +/-/+/- mice had the highest levels of strains tested. To explain the pattern of idiotypic relatedness, 2G7 was tested for anti-DNA activity by ELISA. 2G7 displayed activity for single-stranded DNA as well as synthetic DNA and RNA homopolymers. Absorption analysis indicated that the anti-DNA and anti-Sm binding activities were the product of the same antibody. These results suggest that anti-Sm and anti-DNA may be related by both idiotype and antigenic specificity, providing a mechanism for their common expression in SLE.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1985

Volume

135

Issue

6

Start / End Page

4080 / 4085

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Rabbits
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice, Inbred AKR
 

Citation

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MLA
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Pisetsky, D. S., Hoch, S. O., Klatt, C. L., O’Donnell, M. A., & Keene, J. D. (1985). Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity. J Immunol, 135(6), 4080–4085.
Pisetsky, D. S., S. O. Hoch, C. L. Klatt, M. A. O’Donnell, and J. D. Keene. “Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity.J Immunol 135, no. 6 (December 1985): 4080–85.
Pisetsky DS, Hoch SO, Klatt CL, O’Donnell MA, Keene JD. Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity. J Immunol. 1985 Dec;135(6):4080–5.
Pisetsky, D. S., et al. “Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity.J Immunol, vol. 135, no. 6, Dec. 1985, pp. 4080–85.
Pisetsky DS, Hoch SO, Klatt CL, O’Donnell MA, Keene JD. Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity. J Immunol. 1985 Dec;135(6):4080–4085.

Published In

J Immunol

ISSN

0022-1767

Publication Date

December 1985

Volume

135

Issue

6

Start / End Page

4080 / 4085

Location

United States

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Rabbits
  • Mice, Inbred NZB
  • Mice, Inbred DBA
  • Mice, Inbred CBA
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice, Inbred AKR