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Functional specialization of beta-arrestin interactions revealed by proteomic analysis.

Publication ,  Journal Article
Xiao, K; McClatchy, DB; Shukla, AK; Zhao, Y; Chen, M; Shenoy, SK; Yates, JR; Lefkowitz, RJ
Published in: Proc Natl Acad Sci U S A
July 17, 2007

Beta-arrestins are cytosolic proteins that form complexes with seven-transmembrane receptors after agonist stimulation and phosphorylation by the G protein-coupled receptor kinases. They play an essential role in receptor desensitization and endocytosis, and they also serve as receptor-regulated signaling scaffolds and adaptors. Moreover, in the past decade, a growing list of protein-protein interactions of beta-arrestins pertinent to these functions has been documented. The discovery of several novel functions of beta-arrestins stimulated us to perform a global proteomics analysis of beta-arrestin-interacting proteins (interactome) as modulated by a model seven-transmembrane receptor, the angiotensin II type 1a receptor, in an attempt to assess the full range of functions of these versatile molecules. As determined by LC tandem MS, 71 proteins interacted with beta-arrestin 1, 164 interacted with beta-arrestin 2, and 102 interacted with both beta-arrestins. Some proteins bound only after agonist stimulation, whereas others dissociated. Bioinformatics analysis of the data indicates that proteins involved in cellular signaling, organization, and nucleic acid binding are the most highly represented in the beta-arrestin interactome. Surprisingly, both S-arrestin (visual arrestin) and X-arrestin (cone arrestin) were also found in heteromeric complex with beta-arrestins. The beta-arrestin interactors distribute not only in the cytoplasm, but also in the nucleus as well as other subcellular compartments. The binding of 16 randomly selected newly identified beta-arrestin partners was validated by coimmunoprecipitation assays in HEK293 cells. This study provides a comprehensive analysis of proteins that bind beta-arrestin isoforms and underscores their potentially broad regulatory roles in mammalian cellular physiology.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 17, 2007

Volume

104

Issue

29

Start / End Page

12011 / 12016

Location

United States

Related Subject Headings

  • beta-Arrestins
  • beta-Arrestin 2
  • beta-Arrestin 1
  • Signal Transduction
  • Proteomics
  • Protein Processing, Post-Translational
  • Protein Biosynthesis
  • Protein Binding
  • Mass Spectrometry
  • Immunoprecipitation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Xiao, K., McClatchy, D. B., Shukla, A. K., Zhao, Y., Chen, M., Shenoy, S. K., … Lefkowitz, R. J. (2007). Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proc Natl Acad Sci U S A, 104(29), 12011–12016. https://doi.org/10.1073/pnas.0704849104
Xiao, Kunhong, Daniel B. McClatchy, Arun K. Shukla, Yang Zhao, Minyong Chen, Sudha K. Shenoy, John R. Yates, and Robert J. Lefkowitz. “Functional specialization of beta-arrestin interactions revealed by proteomic analysis.Proc Natl Acad Sci U S A 104, no. 29 (July 17, 2007): 12011–16. https://doi.org/10.1073/pnas.0704849104.
Xiao K, McClatchy DB, Shukla AK, Zhao Y, Chen M, Shenoy SK, et al. Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12011–6.
Xiao, Kunhong, et al. “Functional specialization of beta-arrestin interactions revealed by proteomic analysis.Proc Natl Acad Sci U S A, vol. 104, no. 29, July 2007, pp. 12011–16. Pubmed, doi:10.1073/pnas.0704849104.
Xiao K, McClatchy DB, Shukla AK, Zhao Y, Chen M, Shenoy SK, Yates JR, Lefkowitz RJ. Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12011–12016.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 17, 2007

Volume

104

Issue

29

Start / End Page

12011 / 12016

Location

United States

Related Subject Headings

  • beta-Arrestins
  • beta-Arrestin 2
  • beta-Arrestin 1
  • Signal Transduction
  • Proteomics
  • Protein Processing, Post-Translational
  • Protein Biosynthesis
  • Protein Binding
  • Mass Spectrometry
  • Immunoprecipitation