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Synthetic peptides containing T and B cell epitopes from human immunodeficiency virus envelope gp120 induce anti-HIV proliferative responses and high titers of neutralizing antibodies in rhesus monkeys.

Publication ,  Journal Article
Hart, MK; Palker, TJ; Matthews, TJ; Langlois, AJ; Lerche, NW; Martin, ME; Scearce, RM; McDanal, C; Bolognesi, DP; Haynes, BF
Published in: J Immunol
October 15, 1990

We have previously described a synthetic peptide (T1-SP10) derived from two noncontiguous regions of HTLVIIIB envelope gp120 (T1, amino acids 428-443; SP10, amino acids 303-321) that induced type-specific anti-HIV neutralizing antibodies and T cell proliferative responses against native HIV gp120 when used as a carrier-free immunogen in goats. In this study, HTLVIIIB T1-SP10 synthetic peptides were used to immunize rhesus monkeys to determine if the peptides were capable of eliciting HIV-specific neutralizing antibody and proliferative responses in primates. Four compounds (alum, polyA:polyU, threonyl-muramyldipeptide (MDP) and IFA) were also compared for efficacy as adjuvants in this system. Rhesus monkeys immunized with T1-SP10 peptides generated high titers of antibodies against the immunogens and also against HTLVIIIB gp120. Sera from all four animals given T1-SP10 in IFA or threonyl-MDP neutralized infection by HTLVIIIB and blocked virus-dependent cell fusion events. A peak neutralization titer of 1:940 was seen in one animal given IFA (19600) and a titer of 1:900 was seen in one of the monkeys (17371) given threonyl-MDP. Proliferative responses of immune rhesus PBMC to T1-SP10 appeared after the first injection. After eight immunizations, two of eight monkeys (one injected with peptides in threonyl-MDP and one given peptides in IFA) had PBMC proliferative responses to native HTLVIIIB gp120. These data demonstrate that the carrier-free T1-SP10 synthetic peptide construct can induce high titers of neutralizing anti-HIV antibody responses and PBMC proliferative responses to HIV in primates.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 15, 1990

Volume

145

Issue

8

Start / End Page

2677 / 2685

Location

United States

Related Subject Headings

  • Vaccines, Synthetic
  • T-Lymphocytes
  • Peptides
  • Neutralization Tests
  • Molecular Sequence Data
  • Macaca mulatta
  • Lymphocyte Subsets
  • Lymphocyte Activation
  • Leukocyte Count
  • Immunology
 

Citation

APA
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MLA
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Hart, M. K., Palker, T. J., Matthews, T. J., Langlois, A. J., Lerche, N. W., Martin, M. E., … Haynes, B. F. (1990). Synthetic peptides containing T and B cell epitopes from human immunodeficiency virus envelope gp120 induce anti-HIV proliferative responses and high titers of neutralizing antibodies in rhesus monkeys. J Immunol, 145(8), 2677–2685.
Hart, M. K., T. J. Palker, T. J. Matthews, A. J. Langlois, N. W. Lerche, M. E. Martin, R. M. Scearce, C. McDanal, D. P. Bolognesi, and B. F. Haynes. “Synthetic peptides containing T and B cell epitopes from human immunodeficiency virus envelope gp120 induce anti-HIV proliferative responses and high titers of neutralizing antibodies in rhesus monkeys.J Immunol 145, no. 8 (October 15, 1990): 2677–85.
Hart MK, Palker TJ, Matthews TJ, Langlois AJ, Lerche NW, Martin ME, Scearce RM, McDanal C, Bolognesi DP, Haynes BF. Synthetic peptides containing T and B cell epitopes from human immunodeficiency virus envelope gp120 induce anti-HIV proliferative responses and high titers of neutralizing antibodies in rhesus monkeys. J Immunol. 1990 Oct 15;145(8):2677–2685.

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 15, 1990

Volume

145

Issue

8

Start / End Page

2677 / 2685

Location

United States

Related Subject Headings

  • Vaccines, Synthetic
  • T-Lymphocytes
  • Peptides
  • Neutralization Tests
  • Molecular Sequence Data
  • Macaca mulatta
  • Lymphocyte Subsets
  • Lymphocyte Activation
  • Leukocyte Count
  • Immunology