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Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript.

Publication ,  Journal Article
Begley, CG; Aplan, PD; Davey, MP; Nakahara, K; Tchorz, K; Kurtzberg, J; Hershfield, MS; Haynes, BF; Cohen, DI; Waldmann, TA
Published in: Proc Natl Acad Sci U S A
March 1989

We have studied a leukemic stem-cell line, DU.528, that is able to differentiate into myeloid and lymphoid cells. The leukemic cells have a translocation between chromosomes 1 and 14, t(1;14)(p33;q11), which we have molecularly cloned and sequenced. Initial screening used joining (J)-segment probes from the T-cell receptor (TCR) alpha- and delta-chain loci. In apparent concert with the translocation, a deletion has occurred between delta-chain diversity (D)-region genes D delta 1 and D delta 2. D delta 2 was observed on derivative chromosome 1 [der(1)] and D delta 1 on der(14) with a deletion of the intervening 10 kilobases of germ-line DNA. The nature of the D delta 1-D delta 2 deletional event implicates a lymphoid recombinase in the mechanism of the translocation. As a consequence of the translocation, an unusual fusion transcript was generated. Probes from chromosome 1 detected a previously unreported transcript in RNA from both the cell line and the patient. A chromosome 14 probe identified the same transcript, thus confirming a fusion transcript derived from both chromosomes 1 and 14. This translocation may identify a gene for which we propose the name SCL (stem-cell leukemia) that is important for hemopoietic development and oncogenesis and that has been disrupted or altered in this stem-cell line.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1989

Volume

86

Issue

6

Start / End Page

2031 / 2035

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Translocation, Genetic
  • Transcription, Genetic
  • Stem Cells
  • Receptors, Antigen, T-Cell
  • RNA
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Leukemia, Myeloid
  • Leukemia, Lymphoid
 

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Begley, C. G., Aplan, P. D., Davey, M. P., Nakahara, K., Tchorz, K., Kurtzberg, J., … Waldmann, T. A. (1989). Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript. Proc Natl Acad Sci U S A, 86(6), 2031–2035. https://doi.org/10.1073/pnas.86.6.2031
Begley, C. G., P. D. Aplan, M. P. Davey, K. Nakahara, K. Tchorz, J. Kurtzberg, M. S. Hershfield, B. F. Haynes, D. I. Cohen, and T. A. Waldmann. “Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript.Proc Natl Acad Sci U S A 86, no. 6 (March 1989): 2031–35. https://doi.org/10.1073/pnas.86.6.2031.
Begley CG, Aplan PD, Davey MP, Nakahara K, Tchorz K, Kurtzberg J, et al. Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript. Proc Natl Acad Sci U S A. 1989 Mar;86(6):2031–5.
Begley, C. G., et al. “Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript.Proc Natl Acad Sci U S A, vol. 86, no. 6, Mar. 1989, pp. 2031–35. Pubmed, doi:10.1073/pnas.86.6.2031.
Begley CG, Aplan PD, Davey MP, Nakahara K, Tchorz K, Kurtzberg J, Hershfield MS, Haynes BF, Cohen DI, Waldmann TA. Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript. Proc Natl Acad Sci U S A. 1989 Mar;86(6):2031–2035.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1989

Volume

86

Issue

6

Start / End Page

2031 / 2035

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Translocation, Genetic
  • Transcription, Genetic
  • Stem Cells
  • Receptors, Antigen, T-Cell
  • RNA
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Leukemia, Myeloid
  • Leukemia, Lymphoid