Scholars@Duke publication: Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.

Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.

Publication , Journal Article

Haynes, BF; Ma, B; Montefiori, DC; Wrin, T; Petropoulos, CJ; Sutherland, LL; Scearce, RM; Denton, C; Xia, S-M; Korber, BT; Liao, H-X

Published in: Virology

February 5, 2006

Published version (DOI) Link to item

The HIV-1 gp120 V3 loop is a potent inducer of neutralizing antibodies for T cell line adapted-HIV-1, but less so for primary isolates. We hypothesized that peptides representative of the diversity of natural HIV-1 V3 loop variants might capture elements of conserved higher order structures and so stimulate broadly reactive neutralizing antibodies. We designed a panel of 29 subtype B V3 sequences postulated to reflect the range of V3 diversity. These peptides were used to immunize guinea pigs. The most effective peptide (62.19) clustered around the subtype B consensus sequence and induced antibodies that reproducibly neutralized 31% of the subtype B HIV-1 primary isolates evaluated, but exhibited limited cross-neutralization of non-subtype B HIV-1 strains. Taken together, these data demonstrated that the limited neutralization profile of antibodies induced by optimal subtype B V3 motifs likely represents the maximum breadth of neutralization of subtype B HIV-1 primary isolates attainable by anti-V3 peptide antibodies.

Duke Scholars

Author David Charles Montefiori Surgery, Surgical Sciences

Author Barton Ford Haynes Medicine, Duke Human Vaccine Institute

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Published In

Virology

DOI

10.1016/j.virol.2005.08.042

ISSN

0042-6822

Publication Date

February 5, 2006

Volume

345

Issue

Start / End Page

44 / 55

Location

United States

Related Subject Headings

Virology
Sequence Homology, Amino Acid
Peptide Fragments
Neutralization Tests
Humans
HIV-1
HIV Envelope Protein gp120
Guinea Pigs
Cross Reactions
Consensus Sequence

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NLM

Haynes, B. F., Ma, B., Montefiori, D. C., Wrin, T., Petropoulos, C. J., Sutherland, L. L., … Liao, H.-X. (2006). Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates. Virology, 345(1), 44–55. https://doi.org/10.1016/j.virol.2005.08.042

Haynes, Barton F., Benjiang Ma, David C. Montefiori, Terri Wrin, Christos J. Petropoulos, Laura L. Sutherland, Richard M. Scearce, et al. “Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.” Virology 345, no. 1 (February 5, 2006): 44–55. https://doi.org/10.1016/j.virol.2005.08.042.

Haynes BF, Ma B, Montefiori DC, Wrin T, Petropoulos CJ, Sutherland LL, et al. Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates. Virology. 2006 Feb 5;345(1):44–55.

Haynes, Barton F., et al. “Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates.” Virology, vol. 345, no. 1, Feb. 2006, pp. 44–55. Pubmed, doi:10.1016/j.virol.2005.08.042.

Haynes BF, Ma B, Montefiori DC, Wrin T, Petropoulos CJ, Sutherland LL, Scearce RM, Denton C, Xia S-M, Korber BT, Liao H-X. Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates. Virology. 2006 Feb 5;345(1):44–55.