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Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae.

Publication ,  Journal Article
Magwene, PM; Kayıkçı, Ö; Granek, JA; Reininga, JM; Scholl, Z; Murray, D
Published in: Proc Natl Acad Sci U S A
February 1, 2011

We carried out a population genomic survey of Saccharomyces cerevisiae diploid isolates and find that many budding yeast strains have high levels of genomic heterozygosity, much of which is likely due to outcrossing. We demonstrate that variation in heterozygosity among strains is correlated with a life-history trade-off that involves how readily yeast switch from asexual to sexual reproduction under nutrient stress. This trade-off is reflected in a negative relationship between sporulation efficiency and pseudohyphal development and correlates with variation in the expression of RME1, a transcription factor with pleiotropic effects on meiosis and filamentous growth. Selection for alternate life-history strategies in natural versus human-associated environments likely contributes to differential maintenance of genomic heterozygosity through its effect on the frequency that yeast lineages experience sexual cycles and hence the opportunity for inbreeding. In addition to elevated levels of heterozygosity, many strains exhibit large genomic regions of loss-of-heterozygosity (LOH), suggesting that mitotic recombination has a significant impact on genetic variation in this species. This study provides new insights into the roles that both outcrossing and mitotic recombination play in shaping the genome architecture of Saccharomyces cerevisiae. This study also provides a unique case where stark differences in the genomic distribution of genetic variation among individuals of the same species can be largely explained by a life-history trade-off.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 1, 2011

Volume

108

Issue

5

Start / End Page

1987 / 1992

Location

United States

Related Subject Headings

  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Mitosis
  • Loss of Heterozygosity
  • Genome, Fungal
  • Evolution, Molecular
 

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Magwene, P. M., Kayıkçı, Ö., Granek, J. A., Reininga, J. M., Scholl, Z., & Murray, D. (2011). Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 108(5), 1987–1992. https://doi.org/10.1073/pnas.1012544108
Magwene, Paul M., Ömür Kayıkçı, Joshua A. Granek, Jennifer M. Reininga, Zackary Scholl, and Debra Murray. “Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae.Proc Natl Acad Sci U S A 108, no. 5 (February 1, 2011): 1987–92. https://doi.org/10.1073/pnas.1012544108.
Magwene PM, Kayıkçı Ö, Granek JA, Reininga JM, Scholl Z, Murray D. Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1987–92.
Magwene, Paul M., et al. “Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae.Proc Natl Acad Sci U S A, vol. 108, no. 5, Feb. 2011, pp. 1987–92. Pubmed, doi:10.1073/pnas.1012544108.
Magwene PM, Kayıkçı Ö, Granek JA, Reininga JM, Scholl Z, Murray D. Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1987–1992.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

February 1, 2011

Volume

108

Issue

5

Start / End Page

1987 / 1992

Location

United States

Related Subject Headings

  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Mitosis
  • Loss of Heterozygosity
  • Genome, Fungal
  • Evolution, Molecular