Skip to main content

The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.

Publication ,  Journal Article
Cox, GM; Rude, TH; Dykstra, CC; Perfect, JR
Published in: J Med Vet Mycol
1995

Using heterologous probing of a genomic library, we have cloned and sequenced the actin gene from the pathogenic yeast Cryptococcus neoformans. The actin gene is 1371 bp in length, and exists as a single copy, as is the case for all fungi studied to date. The locations of the introns in the C. neoformans actin gene are unique among all other known actin genes, and the deduced coding sequence results in a 375 amino acid chain with very high homology to other actins. A phylogenetic tree comprising 31 actin-coding sequences from a wide variety of organisms shows that the C. neoformans actin gene is grouped on a distinct branch together with all other known fungal actin sequences. The availability of the C. neoformans actin gene will aid future phylogenetic and molecular studies of this important human pathogen.

Duke Scholars

Published In

J Med Vet Mycol

DOI

ISSN

0268-1218

Publication Date

1995

Volume

33

Issue

4

Start / End Page

261 / 266

Location

England

Related Subject Headings

  • Vertebrates
  • Plants
  • Phylogeny
  • Mycology & Parasitology
  • Molecular Sequence Data
  • Karyotyping
  • Invertebrates
  • Humans
  • Genomic Library
  • Genes, Fungal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cox, G. M., Rude, T. H., Dykstra, C. C., & Perfect, J. R. (1995). The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis. J Med Vet Mycol, 33(4), 261–266. https://doi.org/10.1080/02681219580000521
Cox, G. M., T. H. Rude, C. C. Dykstra, and J. R. Perfect. “The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.J Med Vet Mycol 33, no. 4 (1995): 261–66. https://doi.org/10.1080/02681219580000521.
Cox GM, Rude TH, Dykstra CC, Perfect JR. The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis. J Med Vet Mycol. 1995;33(4):261–6.
Cox, G. M., et al. “The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.J Med Vet Mycol, vol. 33, no. 4, 1995, pp. 261–66. Pubmed, doi:10.1080/02681219580000521.
Cox GM, Rude TH, Dykstra CC, Perfect JR. The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis. J Med Vet Mycol. 1995;33(4):261–266.

Published In

J Med Vet Mycol

DOI

ISSN

0268-1218

Publication Date

1995

Volume

33

Issue

4

Start / End Page

261 / 266

Location

England

Related Subject Headings

  • Vertebrates
  • Plants
  • Phylogeny
  • Mycology & Parasitology
  • Molecular Sequence Data
  • Karyotyping
  • Invertebrates
  • Humans
  • Genomic Library
  • Genes, Fungal