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The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer.

Publication ,  Journal Article
Gottlin, EB; Bentley, RC; Campa, MJ; Pisetsky, DS; Herndon, JE; Patz, EF
Published in: J Thorac Oncol
October 2011

INTRODUCTION: Lung cancers can display immune cell infiltration although the role of an adaptive immune response in disease pathogenesis is unknown. To investigate the possibility of a functional humoral response to the tumor, we surveyed histologic sections from non-small cell lung cancer (NSCLC) tumors for germinal centers (GCs) and assessed whether there was an association between the presence of GCs and tumor stage. METHODS: Tumor sections from 91 patients with all stages of NSCLC were examined by a pathologist blinded to clinical data. GCs were identified by hematoxylin and eosin staining patterns and confirmed by immunohistochemical staining for B-cell markers, BCL-6 and CD21. The distribution of GCs within the tumor or the tumor margin was recorded. Statistical analysis was performed to evaluate the association between stage and presence of GCs. RESULTS: Thirty-five percent of all tumors evaluated contained GCs, and sections evaluated by immunohistochemistry showed positive staining for both B-cell markers. GCs were seen both within the tumor and the tumor margin, consistent with an immune response to antigen stimulation. Patients with stage I NSCLC had a higher prevalence of intratumoral GCs than patients with stages II to IV (Cochran-Armitage Trend Test p = 0.02011). There was no association of stage with GCs in the tumor margin. CONCLUSIONS: Intratumoral GCs are associated with early-stage NSCLC. Further characterization of intratumoral GCs may lead to new diagnostic and therapeutic strategies based on manipulation of the adaptive immune response.

Duke Scholars

Published In

J Thorac Oncol

DOI

EISSN

1556-1380

Publication Date

October 2011

Volume

6

Issue

10

Start / End Page

1687 / 1690

Location

United States

Related Subject Headings

  • Proto-Oncogene Proteins c-bcl-2
  • Prognosis
  • Oncology & Carcinogenesis
  • Middle Aged
  • Male
  • Lung Neoplasms
  • Immunoenzyme Techniques
  • Humans
  • Germinal Center
  • Follow-Up Studies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gottlin, E. B., Bentley, R. C., Campa, M. J., Pisetsky, D. S., Herndon, J. E., & Patz, E. F. (2011). The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer. J Thorac Oncol, 6(10), 1687–1690. https://doi.org/10.1097/JTO.0b013e3182217bec
Gottlin, Elizabeth B., Rex C. Bentley, Michael J. Campa, David S. Pisetsky, James E. Herndon, and Edward F. Patz. “The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer.J Thorac Oncol 6, no. 10 (October 2011): 1687–90. https://doi.org/10.1097/JTO.0b013e3182217bec.
Gottlin EB, Bentley RC, Campa MJ, Pisetsky DS, Herndon JE, Patz EF. The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer. J Thorac Oncol. 2011 Oct;6(10):1687–90.
Gottlin, Elizabeth B., et al. “The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer.J Thorac Oncol, vol. 6, no. 10, Oct. 2011, pp. 1687–90. Pubmed, doi:10.1097/JTO.0b013e3182217bec.
Gottlin EB, Bentley RC, Campa MJ, Pisetsky DS, Herndon JE, Patz EF. The Association of Intratumoral Germinal Centers with early-stage non-small cell lung cancer. J Thorac Oncol. 2011 Oct;6(10):1687–1690.
Journal cover image

Published In

J Thorac Oncol

DOI

EISSN

1556-1380

Publication Date

October 2011

Volume

6

Issue

10

Start / End Page

1687 / 1690

Location

United States

Related Subject Headings

  • Proto-Oncogene Proteins c-bcl-2
  • Prognosis
  • Oncology & Carcinogenesis
  • Middle Aged
  • Male
  • Lung Neoplasms
  • Immunoenzyme Techniques
  • Humans
  • Germinal Center
  • Follow-Up Studies