Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins.
Publication
, Journal Article
Vaidyanathan, G; Zalutsky, MR
Published in: Nat Protoc
2006
A procedure for the synthesis of N-succinimidyl 3-iodobenzoate labeled with any iodine isotope ([*I]SIB), which is an agent used in the radioiodination of proteins and peptides, from its tin precursor N-succinimidyl 3-(tri-n-butylstannyl)benzoate (STB) is described. Also included are protocols for the synthesis of an unlabeled standard of SIB and the tin precursor. Radioiododestannylation of STB using tert-butylhydroperoxide as the oxidant gives [*I]SIB in 80% radiochemical yields. The total time for the synthesis of [*I]SIB from STB is approximately 95 min. Use of [*I]SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method.
Duke Scholars
Published In
Nat Protoc
DOI
EISSN
1750-2799
Publication Date
2006
Volume
1
Issue
2
Start / End Page
707 / 713
Location
England
Related Subject Headings
- Proteins
- Molecular Structure
- Isotope Labeling
- Iodobenzoates
- Iodine Radioisotopes
- Bioinformatics
- 11 Medical and Health Sciences
- 06 Biological Sciences
- 03 Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Vaidyanathan, G., & Zalutsky, M. R. (2006). Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins. Nat Protoc, 1(2), 707–713. https://doi.org/10.1038/nprot.2006.99
Vaidyanathan, Ganesan, and Michael R. Zalutsky. “Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins.” Nat Protoc 1, no. 2 (2006): 707–13. https://doi.org/10.1038/nprot.2006.99.
Vaidyanathan G, Zalutsky MR. Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins. Nat Protoc. 2006;1(2):707–13.
Vaidyanathan, Ganesan, and Michael R. Zalutsky. “Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins.” Nat Protoc, vol. 1, no. 2, 2006, pp. 707–13. Pubmed, doi:10.1038/nprot.2006.99.
Vaidyanathan G, Zalutsky MR. Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins. Nat Protoc. 2006;1(2):707–713.
Published In
Nat Protoc
DOI
EISSN
1750-2799
Publication Date
2006
Volume
1
Issue
2
Start / End Page
707 / 713
Location
England
Related Subject Headings
- Proteins
- Molecular Structure
- Isotope Labeling
- Iodobenzoates
- Iodine Radioisotopes
- Bioinformatics
- 11 Medical and Health Sciences
- 06 Biological Sciences
- 03 Chemical Sciences