A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.
Publication
, Journal Article
Farmer, BT; Venters, RA; Spicer, LD; Wittekind, MG; Müller, L
Published in: J Biomol NMR
March 1992
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
Duke Scholars
Published In
J Biomol NMR
DOI
ISSN
0925-2738
Publication Date
March 1992
Volume
2
Issue
2
Start / End Page
195 / 202
Location
Netherlands
Related Subject Headings
- Protein Conformation
- Nitrogen Isotopes
- Mathematics
- Magnetic Resonance Spectroscopy
- Isoenzymes
- Humans
- Carbonic Anhydrases
- Carbon Isotopes
- Biophysics
- 51 Physical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Farmer, B. T., Venters, R. A., Spicer, L. D., Wittekind, M. G., & Müller, L. (1992). A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules. J Biomol NMR, 2(2), 195–202. https://doi.org/10.1007/BF01875530
Farmer, B. T., R. A. Venters, L. D. Spicer, M. G. Wittekind, and L. Müller. “A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.” J Biomol NMR 2, no. 2 (March 1992): 195–202. https://doi.org/10.1007/BF01875530.
Farmer BT, Venters RA, Spicer LD, Wittekind MG, Müller L. A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules. J Biomol NMR. 1992 Mar;2(2):195–202.
Farmer, B. T., et al. “A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.” J Biomol NMR, vol. 2, no. 2, Mar. 1992, pp. 195–202. Pubmed, doi:10.1007/BF01875530.
Farmer BT, Venters RA, Spicer LD, Wittekind MG, Müller L. A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules. J Biomol NMR. 1992 Mar;2(2):195–202.
Published In
J Biomol NMR
DOI
ISSN
0925-2738
Publication Date
March 1992
Volume
2
Issue
2
Start / End Page
195 / 202
Location
Netherlands
Related Subject Headings
- Protein Conformation
- Nitrogen Isotopes
- Mathematics
- Magnetic Resonance Spectroscopy
- Isoenzymes
- Humans
- Carbonic Anhydrases
- Carbon Isotopes
- Biophysics
- 51 Physical sciences