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Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients.

Publication ,  Journal Article
Steinbach, WJ; Addison, RM; McLaughlin, L; Gerrald, Q; Martin, PL; Driscoll, T; Bentsen, C; Perfect, JR; Alexander, BD
Published in: Pediatr Infect Dis J
July 2007

BACKGROUND: The galactomannan (GM) assay is an approved noninvasive test for detection of invasive aspergillosis (IA) that has been validated in adult patients with hematologic malignancies who are undergoing bone marrow transplantation. There have been few studies with this assay in pediatric patients, but early reports suggest that there may be differences in the performance such that false-positive GM tests in pediatric patients are more common than in adult patients. METHODS: We performed a prospective study in pediatric hematopoietic stem cell transplant recipients with twice-weekly sampling for GM detection during the highest risk periods of neutropenia and graft-versus-host disease. We analyzed 826 serum samples from 64 patients, including 15 serum samples from one patient diagnosed with probable IA according to defined criteria. RESULTS: Twenty of 811 samples tested positive on repeat testing (specificity, 97.5%; 95% CI: 96.2-98.4%) including samples from 8 of 63 patients without clinical evidence of IA according to study criteria (specificity, 87.3%; 95% CI: 76.9-93.4%). Eleven patients received piperacillin/tazobactam therapy, and 4 of the 11 patients had a positive assay result coinciding with the dates of piperacillin/tazobactam administration. When samples from these patients were excluded, specificity increased to 98.4% (95% CI: 97.2-99.1%) by sample and to 91.5% (95% CI: 81.6-96.3%) by patient. CONCLUSIONS: The GM assay holds promise for early, noninvasive diagnosis of IA in high-risk children and false-positive results were not common or unexplainable. This study supports further validation of this assay in a large-scale, pediatric-dedicated format.

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Published In

Pediatr Infect Dis J

DOI

ISSN

0891-3668

Publication Date

July 2007

Volume

26

Issue

7

Start / End Page

558 / 564

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Pediatrics
  • Mannans
  • Humans
  • Hematopoietic Stem Cell Transplantation
  • Galactose
  • Enzyme-Linked Immunosorbent Assay
  • Child
  • Aspergillus
 

Citation

APA
Chicago
ICMJE
MLA
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Steinbach, W. J., Addison, R. M., McLaughlin, L., Gerrald, Q., Martin, P. L., Driscoll, T., … Alexander, B. D. (2007). Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients. Pediatr Infect Dis J, 26(7), 558–564. https://doi.org/10.1097/INF.0b013e3180616cbb
Steinbach, William J., Rachel M. Addison, Lisa McLaughlin, Quincy Gerrald, Paul L. Martin, Timothy Driscoll, Christopher Bentsen, John R. Perfect, and Barbara D. Alexander. “Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients.Pediatr Infect Dis J 26, no. 7 (July 2007): 558–64. https://doi.org/10.1097/INF.0b013e3180616cbb.
Steinbach WJ, Addison RM, McLaughlin L, Gerrald Q, Martin PL, Driscoll T, et al. Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients. Pediatr Infect Dis J. 2007 Jul;26(7):558–64.
Steinbach, William J., et al. “Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients.Pediatr Infect Dis J, vol. 26, no. 7, July 2007, pp. 558–64. Pubmed, doi:10.1097/INF.0b013e3180616cbb.
Steinbach WJ, Addison RM, McLaughlin L, Gerrald Q, Martin PL, Driscoll T, Bentsen C, Perfect JR, Alexander BD. Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients. Pediatr Infect Dis J. 2007 Jul;26(7):558–564.

Published In

Pediatr Infect Dis J

DOI

ISSN

0891-3668

Publication Date

July 2007

Volume

26

Issue

7

Start / End Page

558 / 564

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Pediatrics
  • Mannans
  • Humans
  • Hematopoietic Stem Cell Transplantation
  • Galactose
  • Enzyme-Linked Immunosorbent Assay
  • Child
  • Aspergillus