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Induction of IL-6 expression by mechanical stress in the trabecular meshwork.

Publication ,  Journal Article
Liton, PB; Luna, C; Bodman, M; Hong, A; Epstein, DL; Gonzalez, P
Published in: Biochem Biophys Res Commun
December 2, 2005

The trabecular meshwork (TM)/Schlemm's canal (SC) outflow pathway is the tissue responsible for maintaining normal levels of intraocular pressure. In the present study, we investigate the effects of mechanical stress on the expression of IL-6 in the TM meshwork, as well as the effects of this cytokine on outflow pathway function. Application of cyclic mechanical stress to human TM primary cultures resulted in a statistically significant increase in both secretion and transcription of IL-6, compared to nonstressed controls. Addition of TGF-beta1, which has been reported to be upregulated in TM cells under mechanical stress, also induced a significant activation of both the transcription and secretion of IL-6. Moreover, anti-TGF-b1 antibodies partially blocked the stretch-induced IL-6 production. Injection of IL-6 into perfused porcine anterior segments resulted in a 30% increase in outflow facility, as well as increased permeability through SC cell monolayers. These results suggest a role for IL-6 in the homeostatic modulation of aqueous humor outflow resistance.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 2, 2005

Volume

337

Issue

4

Start / End Page

1229 / 1236

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Trabecular Meshwork
  • Swine
  • Stress, Mechanical
  • Promoter Regions, Genetic
  • Interleukin-6
  • Humans
  • Gene Expression Regulation
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liton, P. B., Luna, C., Bodman, M., Hong, A., Epstein, D. L., & Gonzalez, P. (2005). Induction of IL-6 expression by mechanical stress in the trabecular meshwork. Biochem Biophys Res Commun, 337(4), 1229–1236. https://doi.org/10.1016/j.bbrc.2005.09.182
Liton, Paloma B., Coralia Luna, Marc Bodman, Anna Hong, David L. Epstein, and Pedro Gonzalez. “Induction of IL-6 expression by mechanical stress in the trabecular meshwork.Biochem Biophys Res Commun 337, no. 4 (December 2, 2005): 1229–36. https://doi.org/10.1016/j.bbrc.2005.09.182.
Liton PB, Luna C, Bodman M, Hong A, Epstein DL, Gonzalez P. Induction of IL-6 expression by mechanical stress in the trabecular meshwork. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1229–36.
Liton, Paloma B., et al. “Induction of IL-6 expression by mechanical stress in the trabecular meshwork.Biochem Biophys Res Commun, vol. 337, no. 4, Dec. 2005, pp. 1229–36. Pubmed, doi:10.1016/j.bbrc.2005.09.182.
Liton PB, Luna C, Bodman M, Hong A, Epstein DL, Gonzalez P. Induction of IL-6 expression by mechanical stress in the trabecular meshwork. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1229–1236.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 2, 2005

Volume

337

Issue

4

Start / End Page

1229 / 1236

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Trabecular Meshwork
  • Swine
  • Stress, Mechanical
  • Promoter Regions, Genetic
  • Interleukin-6
  • Humans
  • Gene Expression Regulation
  • Cells, Cultured