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The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation.

Publication ,  Journal Article
Hollingsworth, JW; Chen, BJ; Brass, DM; Berman, K; Gunn, MD; Cook, DN; Schwartz, DA
Published in: Am J Respir Crit Care Med
April 15, 2005

Rapid and selective recruitment of neutrophils into the airspace in response to LPS facilitates the clearance of bacterial pathogens. However, neutrophil infiltration can also participate in the development and progression of environmental airway disease. Previous data have revealed that Toll-like receptor 4 (tlr4) is required for neutrophil recruitment to the lung after either inhaled or systemically administrated LPS from Escherichia coli. Although many cell types express tlr4, endothelial cell expression of tlr4 is specifically required to sequester neutrophils in the lung in response to systemic endotoxin. To identify the cell types requiring trl4 expression for neutrophil recruitment after inhaled LPS, we generated chimeric mice separately expressing tlr4 on either hematopoietic cells or on structural lung cells. Neutrophil recruitment into the airspace was completely restored in tlr4-deficient mice receiving wild-type bone marrow. By contrast, wild-type animals receiving tlr4-deficient marrow had dramatically reduced neutrophil recruitment. Moreover, adoptive transfer of wild-type alveolar macrophages also restored the ability of tlr4-deficient recipient mice to recruit neutrophils to the lung. These data demonstrate the critical role of hematopoietic cells and alveolar macrophages in initiating LPS-induced neutrophil recruitment from the vascular space to the airspace.

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Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

April 15, 2005

Volume

171

Issue

8

Start / End Page

806 / 813

Location

United States

Related Subject Headings

  • Toll-Like Receptor 4
  • Respiratory System
  • Pulmonary Alveoli
  • Pneumonia, Bacterial
  • Peroxidase
  • Neutrophil Infiltration
  • Mice, Inbred C57BL
  • Mice
  • Macrophages, Alveolar
  • Lymphocyte Count
 

Citation

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Hollingsworth, J. W., Chen, B. J., Brass, D. M., Berman, K., Gunn, M. D., Cook, D. N., & Schwartz, D. A. (2005). The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation. Am J Respir Crit Care Med, 171(8), 806–813. https://doi.org/10.1164/rccm.200407-953OC
Hollingsworth, John W., Benny J. Chen, David M. Brass, Katie Berman, M Dee Gunn, Donald N. Cook, and David A. Schwartz. “The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation.Am J Respir Crit Care Med 171, no. 8 (April 15, 2005): 806–13. https://doi.org/10.1164/rccm.200407-953OC.
Hollingsworth JW, Chen BJ, Brass DM, Berman K, Gunn MD, Cook DN, et al. The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation. Am J Respir Crit Care Med. 2005 Apr 15;171(8):806–13.
Hollingsworth, John W., et al. “The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation.Am J Respir Crit Care Med, vol. 171, no. 8, Apr. 2005, pp. 806–13. Pubmed, doi:10.1164/rccm.200407-953OC.
Hollingsworth JW, Chen BJ, Brass DM, Berman K, Gunn MD, Cook DN, Schwartz DA. The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation. Am J Respir Crit Care Med. 2005 Apr 15;171(8):806–813.

Published In

Am J Respir Crit Care Med

DOI

ISSN

1073-449X

Publication Date

April 15, 2005

Volume

171

Issue

8

Start / End Page

806 / 813

Location

United States

Related Subject Headings

  • Toll-Like Receptor 4
  • Respiratory System
  • Pulmonary Alveoli
  • Pneumonia, Bacterial
  • Peroxidase
  • Neutrophil Infiltration
  • Mice, Inbred C57BL
  • Mice
  • Macrophages, Alveolar
  • Lymphocyte Count