Scholars@Duke publication: The protein expression landscape of the Arabidopsis root.

The protein expression landscape of the Arabidopsis root.

Publication , Journal Article

Petricka, JJ; Schauer, MA; Megraw, M; Breakfield, NW; Thompson, JW; Georgiev, S; Soderblom, EJ; Ohler, U; Moseley, MA; Grossniklaus, U; Benfey, PN

Published in: Proc Natl Acad Sci U S A

May 1, 2012

Published version (DOI) Link to item

Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.

Duke Scholars

Author J. Will Thompson Pharmacology & Cancer Biology

Author Erik James Soderblom Cell Biology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.1202546109

EISSN

1091-6490

Publication Date

May 1, 2012

Volume

109

Issue

Start / End Page

6811 / 6818

Location

United States

Related Subject Headings

Tandem Mass Spectrometry
RNA, Plant
Proteomics
Proteome
Protein Interaction Mapping
Protein Array Analysis
Plants, Genetically Modified
Plant Roots
Gene Expression Profiling
DNA Primers

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APA

Chicago

ICMJE

MLA

NLM

Petricka, J. J., Schauer, M. A., Megraw, M., Breakfield, N. W., Thompson, J. W., Georgiev, S., … Benfey, P. N. (2012). The protein expression landscape of the Arabidopsis root. Proc Natl Acad Sci U S A, 109(18), 6811–6818. https://doi.org/10.1073/pnas.1202546109

Petricka, Jalean J., Monica A. Schauer, Molly Megraw, Natalie W. Breakfield, J Will Thompson, Stoyan Georgiev, Erik J. Soderblom, et al. “The protein expression landscape of the Arabidopsis root.” Proc Natl Acad Sci U S A 109, no. 18 (May 1, 2012): 6811–18. https://doi.org/10.1073/pnas.1202546109.

Petricka JJ, Schauer MA, Megraw M, Breakfield NW, Thompson JW, Georgiev S, et al. The protein expression landscape of the Arabidopsis root. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6811–8.

Petricka, Jalean J., et al. “The protein expression landscape of the Arabidopsis root.” Proc Natl Acad Sci U S A, vol. 109, no. 18, May 2012, pp. 6811–18. Pubmed, doi:10.1073/pnas.1202546109.

Petricka JJ, Schauer MA, Megraw M, Breakfield NW, Thompson JW, Georgiev S, Soderblom EJ, Ohler U, Moseley MA, Grossniklaus U, Benfey PN. The protein expression landscape of the Arabidopsis root. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6811–6818.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.1202546109

EISSN

1091-6490

Publication Date

May 1, 2012

Volume

109

Issue

Start / End Page

6811 / 6818

Location

United States

Related Subject Headings

Tandem Mass Spectrometry
RNA, Plant
Proteomics
Proteome
Protein Interaction Mapping
Protein Array Analysis
Plants, Genetically Modified
Plant Roots
Gene Expression Profiling
DNA Primers