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Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas.

Publication ,  Journal Article
Moding, EJ; Clark, DP; Qi, Y; Li, Y; Ma, Y; Ghaghada, K; Johnson, GA; Kirsch, DG; Badea, CT
Published in: Int J Radiat Oncol Biol Phys
April 1, 2013

PURPOSE: To evaluate the effects of radiation therapy on primary tumor vasculature using dual-energy (DE) micro-computed tomography (micro-CT). METHODS AND MATERIALS: Primary sarcomas were generated with mutant Kras and p53. Unirradiated tumors were compared with tumors irradiated with 20 Gy. A liposomal-iodinated contrast agent was administered 1 day after treatment, and mice were imaged immediately after injection (day 1) and 3 days later (day 4) with DE micro-CT. CT-derived tumor sizes were used to assess tumor growth. After DE decomposition, iodine maps were used to assess tumor fractional blood volume (FBV) at day 1 and tumor vascular permeability at day 4. For comparison, tumor vascularity and vascular permeability were also evaluated histologically by use of CD31 immunofluorescence and fluorescently-labeled dextrans. RESULTS: Radiation treatment significantly decreased tumor growth from day 1 to day 4 (P<.05). There was a positive correlation between CT measurement of tumor FBV on day 1 and extravasated iodine on day 4 with microvascular density (MVD) on day 4 (R(2)=0.53) and dextran accumulation (R(2)=0.63) on day 4, respectively. Despite no change in MVD measured by histology, tumor FBV significantly increased after irradiation as measured by DE micro-CT (0.070 vs 0.091, P<.05). Both dextran and liposomal-iodine accumulation in tumors increased significantly after irradiation, with dextran fractional area increasing 5.2-fold and liposomal-iodine concentration increasing 4.0-fold. CONCLUSIONS: DE micro-CT is an effective tool for noninvasive assessment of vascular changes in primary tumors. Tumor blood volume and vascular permeability increased after a single therapeutic dose of radiation treatment.

Duke Scholars

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

April 1, 2013

Volume

85

Issue

5

Start / End Page

1353 / 1359

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Tumor Burden
  • Sarcoma
  • Radiation Dosage
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Perfusion Imaging
  • Oncology & Carcinogenesis
  • Nanoparticles
  • Mice
  • Liposomes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Moding, E. J., Clark, D. P., Qi, Y., Li, Y., Ma, Y., Ghaghada, K., … Badea, C. T. (2013). Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas. Int J Radiat Oncol Biol Phys, 85(5), 1353–1359. https://doi.org/10.1016/j.ijrobp.2012.09.027
Moding, Everett J., Darin P. Clark, Yi Qi, Yifan Li, Yan Ma, Ketan Ghaghada, G Allan Johnson, David G. Kirsch, and Cristian T. Badea. “Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas.Int J Radiat Oncol Biol Phys 85, no. 5 (April 1, 2013): 1353–59. https://doi.org/10.1016/j.ijrobp.2012.09.027.
Moding EJ, Clark DP, Qi Y, Li Y, Ma Y, Ghaghada K, et al. Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas. Int J Radiat Oncol Biol Phys. 2013 Apr 1;85(5):1353–9.
Moding, Everett J., et al. “Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas.Int J Radiat Oncol Biol Phys, vol. 85, no. 5, Apr. 2013, pp. 1353–59. Pubmed, doi:10.1016/j.ijrobp.2012.09.027.
Moding EJ, Clark DP, Qi Y, Li Y, Ma Y, Ghaghada K, Johnson GA, Kirsch DG, Badea CT. Dual-energy micro-computed tomography imaging of radiation-induced vascular changes in primary mouse sarcomas. Int J Radiat Oncol Biol Phys. 2013 Apr 1;85(5):1353–1359.
Journal cover image

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

April 1, 2013

Volume

85

Issue

5

Start / End Page

1353 / 1359

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Tumor Burden
  • Sarcoma
  • Radiation Dosage
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Perfusion Imaging
  • Oncology & Carcinogenesis
  • Nanoparticles
  • Mice
  • Liposomes