Skip to main content
Journal cover image

Heat induces gene amplification in cancer cells.

Publication ,  Journal Article
Yan, B; Ouyang, R; Huang, C; Liu, F; Neill, D; Li, C; Dewhirst, M
Published in: Biochem Biophys Res Commun
October 26, 2012

BACKGROUND: Hyperthermia plays an important role in cancer therapy. However, as with radiation, it can cause DNA damage and therefore genetic instability. We studied whether hyperthermia can induce gene amplification in cancer cells and explored potential underlying molecular mechanisms. MATERIALS AND METHODS: (1) Hyperthermia: HCT116 colon cancer cells received water-submerged heating treatment at 42 or 44°C for 30 min; (2) gene amplification assay using N-(phosphoacetyl)-L-aspartate (PALA) selection of cabamyl-P-synthetase, aspartate transcarbarmylase, dihydro-orotase (cad) gene amplified cells; (3) southern blotting for confirmation of increased cad gene copies in PALA-resistant cells; (4) γH2AX immunostaining to detect γH2AX foci as an indication for DNA double strand breaks. RESULTS: (1) Heat exposure at 42 or 44°C for 30 min induces gene amplification. The frequency of cad gene amplification increased by 2.8 and 6.5 folds respectively; (2) heat exposure at both 42 and 44°C for 30 min induces DNA double strand breaks in HCT116 cells as shown by γH2AX immunostaining. CONCLUSION: This study shows that heat exposure can induce gene amplification in cancer cells, likely through the generation of DNA double strand breaks, which are believed to be required for the initiation of gene amplification. This process may be promoted by heat when cellular proteins that are responsible for checkpoints, DNA replication, DNA repair and telomere functions are denatured. To our knowledge, this is the first study to provide direct evidence of hyperthermia induced gene amplification.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

October 26, 2012

Volume

427

Issue

3

Start / End Page

473 / 477

Location

United States

Related Subject Headings

  • Phosphonoacetic Acid
  • Neoplasms
  • Hyperthermia, Induced
  • Humans
  • Hot Temperature
  • HCT116 Cells
  • Gene Expression Regulation, Neoplastic
  • Gene Amplification
  • Dihydroorotase
  • DNA Breaks, Double-Stranded
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yan, B., Ouyang, R., Huang, C., Liu, F., Neill, D., Li, C., & Dewhirst, M. (2012). Heat induces gene amplification in cancer cells. Biochem Biophys Res Commun, 427(3), 473–477. https://doi.org/10.1016/j.bbrc.2012.09.011
Yan, Bin, Ruoyun Ouyang, Chenghui Huang, Franklin Liu, Daniel Neill, Chuanyuan Li, and Mark Dewhirst. “Heat induces gene amplification in cancer cells.Biochem Biophys Res Commun 427, no. 3 (October 26, 2012): 473–77. https://doi.org/10.1016/j.bbrc.2012.09.011.
Yan B, Ouyang R, Huang C, Liu F, Neill D, Li C, et al. Heat induces gene amplification in cancer cells. Biochem Biophys Res Commun. 2012 Oct 26;427(3):473–7.
Yan, Bin, et al. “Heat induces gene amplification in cancer cells.Biochem Biophys Res Commun, vol. 427, no. 3, Oct. 2012, pp. 473–77. Pubmed, doi:10.1016/j.bbrc.2012.09.011.
Yan B, Ouyang R, Huang C, Liu F, Neill D, Li C, Dewhirst M. Heat induces gene amplification in cancer cells. Biochem Biophys Res Commun. 2012 Oct 26;427(3):473–477.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

October 26, 2012

Volume

427

Issue

3

Start / End Page

473 / 477

Location

United States

Related Subject Headings

  • Phosphonoacetic Acid
  • Neoplasms
  • Hyperthermia, Induced
  • Humans
  • Hot Temperature
  • HCT116 Cells
  • Gene Expression Regulation, Neoplastic
  • Gene Amplification
  • Dihydroorotase
  • DNA Breaks, Double-Stranded