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Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits.

Publication ,  Journal Article
Staats, HF; Fielhauer, JR; Thompson, AL; Tripp, AA; Sobel, AE; Maddaloni, M; Abraham, SN; Pascual, DW
Published in: PLoS One
January 27, 2011

BACKGROUND: We previously reported that the immunogenicity of Hcβtre, a botulinum neurotoxin A (BoNT/A) immunogen, was enhanced by fusion to an epithelial cell binding domain, Ad2F, when nasally delivered to mice with cholera toxin (CT). This study was performed to determine if Ad2F would enhance the nasal immunogenicity of Hcβtre in rabbits, an animal model with a nasal cavity anatomy similar to humans. Since CT is not safe for human use, we also tested the adjuvant activity of compound 48/80 (C48/80), a mast cell activating compound previously determined to safely exhibit nasal adjuvant activity in mice. METHODS: New Zealand White or Dutch Belted rabbits were nasally immunized with Hcβtre or Hcβtre-Ad2F alone or combined with CT or C48/80, and serum samples were tested for the presence of Hcβtre-specific binding (ELISA) or BoNT/A neutralizing antibodies. RESULTS: Hcβtre-Ad2F nasally administered with CT induced serum anti-Hcβtre IgG ELISA and BoNT/A neutralizing antibody titers greater than those induced by Hcβtre + CT. C48/80 provided significant nasal adjuvant activity and induced BoNT/A-neutralizing antibodies similar to those induced by CT. CONCLUSIONS: Ad2F enhanced the nasal immunogenicity of Hcβtre, and the mast cell activator C48/80 was an effective adjuvant for nasal immunization in rabbits, an animal model with a nasal cavity anatomy similar to that in humans.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

January 27, 2011

Volume

6

Issue

1

Start / End Page

e16532

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Rabbits
  • Neurotoxins
  • Mucous Membrane
  • Mice
  • Mast Cells
  • General Science & Technology
  • Drug Delivery Systems
  • Botulinum Toxins, Type A
  • Bacterial Vaccines
 

Citation

APA
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MLA
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Staats, H. F., Fielhauer, J. R., Thompson, A. L., Tripp, A. A., Sobel, A. E., Maddaloni, M., … Pascual, D. W. (2011). Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits. PLoS One, 6(1), e16532. https://doi.org/10.1371/journal.pone.0016532
Staats, Herman F., Jeffrey R. Fielhauer, Afton L. Thompson, Alice A. Tripp, Ashley E. Sobel, Massimo Maddaloni, Soman N. Abraham, and David W. Pascual. “Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits.PLoS One 6, no. 1 (January 27, 2011): e16532. https://doi.org/10.1371/journal.pone.0016532.
Staats HF, Fielhauer JR, Thompson AL, Tripp AA, Sobel AE, Maddaloni M, et al. Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits. PLoS One. 2011 Jan 27;6(1):e16532.
Staats, Herman F., et al. “Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits.PLoS One, vol. 6, no. 1, Jan. 2011, p. e16532. Pubmed, doi:10.1371/journal.pone.0016532.
Staats HF, Fielhauer JR, Thompson AL, Tripp AA, Sobel AE, Maddaloni M, Abraham SN, Pascual DW. Mucosal targeting of a BoNT/A subunit vaccine adjuvanted with a mast cell activator enhances induction of BoNT/A neutralizing antibodies in rabbits. PLoS One. 2011 Jan 27;6(1):e16532.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

January 27, 2011

Volume

6

Issue

1

Start / End Page

e16532

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Rabbits
  • Neurotoxins
  • Mucous Membrane
  • Mice
  • Mast Cells
  • General Science & Technology
  • Drug Delivery Systems
  • Botulinum Toxins, Type A
  • Bacterial Vaccines