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Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex.

Publication ,  Journal Article
Schumacher, MA; Choi, KY; Zalkin, H; Brennan, RG
Published in: J Mol Biol
September 23, 1994
Published version (DOI) Link to item

The purine repressor (PurR) is a DNA-binding protein, which together with a purine corepressor serves to regulate de novo purine and pyrimidine biosynthesis in Escherichia coli. PurR belongs to the structurally homologous lac repressor family of transcription regulators. A PurR-hypoxanthine-DNA complex has been crystallized, with DNA encompassing the high affinity purF operator site and which is 16 base-pairs long with 5'-deoxynucleoside overhangs on each complementary strand. The crystals diffract to better than 2.6 A and take the orthorhombic space group C222(1), with unit cell dimensions a = 175.9 A, b = 94.8 A and c = 81.8 A. The structure determination of this PurR-hypoxanthine-DNA complex will provide the first high resolution view of a Lacl member-DNA complex.

Duke Scholars

Maria Anne Schumacher
Author Maria Anne Schumacher Biochemistry
Richard Gerald Brennan
Author Richard Gerald Brennan Biochemistry

Published In

J Mol Biol

DOI

10.1006/jmbi.1994.1580

ISSN

0022-2836

Publication Date

September 23, 1994

Volume

242

Issue

3

Start / End Page

302 / 305

Location

Netherlands

Related Subject Headings

  • Repressor Proteins
  • Operator Regions, Genetic
  • Molecular Structure
  • Molecular Sequence Data
  • Hypoxanthines
  • Hypoxanthine
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA, Bacterial
  • Crystallography, X-Ray
 

Citation

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MLA
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Schumacher, M. A., Choi, K. Y., Zalkin, H., & Brennan, R. G. (1994). Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. J Mol Biol, 242(3), 302–305. https://doi.org/10.1006/jmbi.1994.1580
Schumacher, M. A., K. Y. Choi, H. Zalkin, and R. G. Brennan. “Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex.” J Mol Biol 242, no. 3 (September 23, 1994): 302–5. https://doi.org/10.1006/jmbi.1994.1580.
Schumacher MA, Choi KY, Zalkin H, Brennan RG. Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. J Mol Biol. 1994 Sep 23;242(3):302–5.
Schumacher, M. A., et al. “Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex.” J Mol Biol, vol. 242, no. 3, Sept. 1994, pp. 302–05. Pubmed, doi:10.1006/jmbi.1994.1580.
Schumacher MA, Choi KY, Zalkin H, Brennan RG. Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. J Mol Biol. 1994 Sep 23;242(3):302–305.
Journal cover image

Published In

J Mol Biol

DOI

10.1006/jmbi.1994.1580

ISSN

0022-2836

Publication Date

September 23, 1994

Volume

242

Issue

3

Start / End Page

302 / 305

Location

Netherlands

Related Subject Headings

  • Repressor Proteins
  • Operator Regions, Genetic
  • Molecular Structure
  • Molecular Sequence Data
  • Hypoxanthines
  • Hypoxanthine
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA, Bacterial
  • Crystallography, X-Ray