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Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS

Publication ,  Journal Article
Young, SP; An, Y; Van Hove, J; Stevens, RD; Chen, YT; Millington, DS
Published in: Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics
December 1, 2002

A hydrophilic interaction liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS/MS) method was established for the separation and analysis of hexose tetrasaccharide linkage isomers in urine, plasma and blood spots. Using the LC-ESI-MS/MS method, the extent of interference from other isomers was also determined. It was found that the tentative identification of isomer 1 as maltotetraose was supported by the increase in both isomer 1 and Glc 4 observed in the plasma of GSD II patients, suggesting it to be derived from glycogen. It was also found that isomer 1 is the major isomer present in blood spot extracts and is probably derived from glycogen stored in the red cells.

Duke Scholars

Sarah Phyllis Young
Author Sarah Phyllis Young Pediatrics, Medical Genetics
Robert David Stevens
Author Robert David Stevens Medicine, Endocrinology, Metabolism, and Nutrition
Yuan-Tsong Chen
Author Yuan-Tsong Chen Pediatrics, Medical Genetics
David Stuart Millington
Author David Stuart Millington Pediatrics, Medical Genetics

Published In

Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics

Publication Date

December 1, 2002

Start / End Page

413 / 414
 

Citation

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Young, S. P., An, Y., Van Hove, J., Stevens, R. D., Chen, Y. T., & Millington, D. S. (2002). Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, 413–414.
Young, S. P., Y. An, J. Van Hove, R. D. Stevens, Y. T. Chen, and D. S. Millington. “Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS.” Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 413–14.
Young SP, An Y, Van Hove J, Stevens RD, Chen YT, Millington DS. Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;413–4.
Young, S. P., et al. “Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS.” Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 413–14.
Young SP, An Y, Van Hove J, Stevens RD, Chen YT, Millington DS. Isomeric oligosaccharide analysis using hydrophilic interaction LC-ESI-MS/MS. Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;413–414.

Published In

Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics

Publication Date

December 1, 2002

Start / End Page

413 / 414