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Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function.

Publication ,  Journal Article
DiLillo, DJ; Weinberg, JB; Yoshizaki, A; Horikawa, M; Bryant, JM; Iwata, Y; Matsushita, T; Matta, KM; Chen, Y; Venturi, GM; Russo, G; Hall, RP ...
Published in: Leukemia
January 2013

Chronic lymphocytic leukemia (CLL) can be immunosuppressive in humans and mice, and CLL cells share multiple phenotypic markers with regulatory B cells that are competent to produce interleukin (IL)-10 (B10 cells). To identify functional links between CLL cells and regulatory B10 cells, the phenotypes and abilities of leukemia cells from 93 patients with overt CLL to express IL-10 were assessed. CD5(+) CLL cells purified from 90% of the patients were IL-10-competent and secreted IL-10 following appropriate ex vivo stimulation. Serum IL-10 levels were also significantly elevated in CLL patients. IL-10-competent cell frequencies were higher among CLLs with IgV(H) mutations, and correlated positively with TCL1 expression. In the TCL1-transgenic (TCL1-Tg) mouse model of CLL, IL-10-competent B cells with the cell surface phenotype of B10 cells expanded significantly with age, preceding the development of overt, CLL-like leukemia. Malignant CLL cells in TCL1-Tg mice also shared immunoregulatory functions with mouse and human B10 cells. Serum IL-10 levels varied in TCL1-Tg mice, but in vivo low-dose lipopolysaccharide treatment induced IL-10 expression in CLL cells and high levels of serum IL-10. Thus, malignant IL-10-competent CLL cells exhibit regulatory functions comparable to normal B10 cells that may contribute to the immunosuppression observed in patients and TCL1-Tg mice.

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Published In

Leukemia

DOI

EISSN

1476-5551

Publication Date

January 2013

Volume

27

Issue

1

Start / End Page

170 / 182

Location

England

Related Subject Headings

  • Proto-Oncogene Proteins
  • Mice, Transgenic
  • Mice
  • Lipopolysaccharides
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Interleukin-10
  • Immunosuppression Therapy
  • Immunology
  • Humans
  • Fluorescent Antibody Technique
 

Citation

APA
Chicago
ICMJE
MLA
NLM
DiLillo, D. J., Weinberg, J. B., Yoshizaki, A., Horikawa, M., Bryant, J. M., Iwata, Y., … Tedder, T. F. (2013). Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function. Leukemia, 27(1), 170–182. https://doi.org/10.1038/leu.2012.165
DiLillo, D. J., J. B. Weinberg, A. Yoshizaki, M. Horikawa, J. M. Bryant, Y. Iwata, T. Matsushita, et al. “Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function.Leukemia 27, no. 1 (January 2013): 170–82. https://doi.org/10.1038/leu.2012.165.
DiLillo DJ, Weinberg JB, Yoshizaki A, Horikawa M, Bryant JM, Iwata Y, et al. Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function. Leukemia. 2013 Jan;27(1):170–82.
DiLillo, D. J., et al. “Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function.Leukemia, vol. 27, no. 1, Jan. 2013, pp. 170–82. Pubmed, doi:10.1038/leu.2012.165.
DiLillo DJ, Weinberg JB, Yoshizaki A, Horikawa M, Bryant JM, Iwata Y, Matsushita T, Matta KM, Chen Y, Venturi GM, Russo G, Gockerman JP, Moore JO, Diehl LF, Volkheimer AD, Friedman DR, Lanasa MC, Hall RP, Tedder TF. Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function. Leukemia. 2013 Jan;27(1):170–182.

Published In

Leukemia

DOI

EISSN

1476-5551

Publication Date

January 2013

Volume

27

Issue

1

Start / End Page

170 / 182

Location

England

Related Subject Headings

  • Proto-Oncogene Proteins
  • Mice, Transgenic
  • Mice
  • Lipopolysaccharides
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Interleukin-10
  • Immunosuppression Therapy
  • Immunology
  • Humans
  • Fluorescent Antibody Technique