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Echinonectin: A new embryonic substrate adhesion protein

Publication ,  Journal Article
Alliegro, MC; Ettensohn, CA; Burdsal, CA; Erickson, HP; McClay, DR
Published in: Journal of Cell Biology
January 1, 1988

An extracellular matrix molecule has been purified from sea urchin (Lytechinus variegatus) embryos. Based on its functional properties and on its origin, this glycoprotein has been given the name 'echinonectin'. Echinonectin is a 230-kD dimer with a unique bow tie shape when viewed by electron microscopy. The molecule is 12 nm long, 8 nm wide at the ends, and narrows to ~4 nm at the middle. It is composed of two 116-kD U-shaped subunits that are attached to each other by disulfide bonds at their respective apices. Polyclonal antibodies were used to localize echinonectin in paraffin-embedded, sectioned specimens by indirect immunofluorescence. The protein is stored in vesicles or granules in unfertilized eggs, is released after fertilization, and later becomes localized on the apical surface of ectoderm cells in the embryo. When used as a substrate in a quantitative in vitro assay, echinonectin is highly effective as an adhesive substrate for dissociated embryonic cells. Because of the quantity, pattern of appearance, distribution, and adhesive characteristics of this protein, we suggest that echinonectin serves as a substrate adhesion molecule during sea urchin development.

Duke Scholars

Published In

Journal of Cell Biology

DOI

ISSN

0021-9525

Publication Date

January 1, 1988

Volume

107

Issue

6 I

Start / End Page

2319 / 2327

Related Subject Headings

  • Developmental Biology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Alliegro, M. C., Ettensohn, C. A., Burdsal, C. A., Erickson, H. P., & McClay, D. R. (1988). Echinonectin: A new embryonic substrate adhesion protein. Journal of Cell Biology, 107(6 I), 2319–2327. https://doi.org/10.1083/jcb.107.6.2319
Alliegro, M. C., C. A. Ettensohn, C. A. Burdsal, H. P. Erickson, and D. R. McClay. “Echinonectin: A new embryonic substrate adhesion protein.” Journal of Cell Biology 107, no. 6 I (January 1, 1988): 2319–27. https://doi.org/10.1083/jcb.107.6.2319.
Alliegro MC, Ettensohn CA, Burdsal CA, Erickson HP, McClay DR. Echinonectin: A new embryonic substrate adhesion protein. Journal of Cell Biology. 1988 Jan 1;107(6 I):2319–27.
Alliegro, M. C., et al. “Echinonectin: A new embryonic substrate adhesion protein.” Journal of Cell Biology, vol. 107, no. 6 I, Jan. 1988, pp. 2319–27. Scopus, doi:10.1083/jcb.107.6.2319.
Alliegro MC, Ettensohn CA, Burdsal CA, Erickson HP, McClay DR. Echinonectin: A new embryonic substrate adhesion protein. Journal of Cell Biology. 1988 Jan 1;107(6 I):2319–2327.

Published In

Journal of Cell Biology

DOI

ISSN

0021-9525

Publication Date

January 1, 1988

Volume

107

Issue

6 I

Start / End Page

2319 / 2327

Related Subject Headings

  • Developmental Biology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences