Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Deep tissue imaging approaches by direct capture of two-photon absorption

Publication ,  Conference
Ye, T; Wagner, W; Tian, P; Yurtsever, G; Fischer, M; Warren, WS
Published in: 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
December 1, 2004

Multiphoton imaging with fluorescence detection has proven to be a powerful method for moderately deep tissue imaging (less than 1 mm) with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or with low efficiency (Melanin). In this paper two femtosecond pulse shaping methods have been demonstrated to detect two-photon absorption (TPA), instead of fluorescence, with high sensitivity, which opens up a wide range of molecular targets. It also facilitates longer excitation wavelengths, which permit greater tissue penetration. Developing the imaging system by using these new methods will be also discussed. © 2004 IEEE.

Duke Scholars

Published In

2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano

ISBN

9780780383883

Publication Date

December 1, 2004

Volume

1

Start / End Page

668 / 671
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ye, T., Wagner, W., Tian, P., Yurtsever, G., Fischer, M., & Warren, W. S. (2004). Deep tissue imaging approaches by direct capture of two-photon absorption. In 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano (Vol. 1, pp. 668–671).
Ye, T., W. Wagner, P. Tian, G. Yurtsever, M. Fischer, and W. S. Warren. “Deep tissue imaging approaches by direct capture of two-photon absorption.” In 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, 1:668–71, 2004.
Ye T, Wagner W, Tian P, Yurtsever G, Fischer M, Warren WS. Deep tissue imaging approaches by direct capture of two-photon absorption. In: 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004. p. 668–71.
Ye, T., et al. “Deep tissue imaging approaches by direct capture of two-photon absorption.” 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, vol. 1, 2004, pp. 668–71.
Ye T, Wagner W, Tian P, Yurtsever G, Fischer M, Warren WS. Deep tissue imaging approaches by direct capture of two-photon absorption. 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004. p. 668–671.

Published In

2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano

ISBN

9780780383883

Publication Date

December 1, 2004

Volume

1

Start / End Page

668 / 671