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Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development.

Publication ,  Journal Article
Que, J; Wilm, B; Hasegawa, H; Wang, F; Bader, D; Hogan, BLM
Published in: Proc Natl Acad Sci U S A
October 28, 2008

During mouse development, the sophisticated vascular network of the lung is established from embryonic day (E) approximately 10.5 and continues to develop postnatally. This network is composed of endothelial cells enclosed by vascular smooth muscle, pericytes, and other mesenchymal cells. Recent in vivo lineage labeling studies in the developing heart and intestine suggest that some of the vascular smooth muscle cells arise from the surface mesothelium. In the developing lung, the Wilm's tumor 1 gene (Wt1) is expressed only in the mesothelial cells. Therefore, we lineage-labeled the mesothelium in vivo by using a Wt1-Cre transgene in combination with either Rosa26R(lacZ), Rosa26R(CAG-hPLAP), or Rosa26R(EYFP) reporter alleles. In all three cases, cells derived from lineage-labeled mesothelium are found inside the lung and as smooth muscle actin (SMA) and PDGF receptor-beta positive cells in the walls of pulmonary blood vessels. To corroborate this finding, we used 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester "mixed isomers" (CCFSE) dye to label mesothelial cells on the surface of the embryonic lung. Over the course of 72-h culture, dye-labeled cells also appear within the lung mesenchyme. Together, our data provide evidence that mesothelial cells serve as a source of vascular smooth muscle cells in the developing lung and suggest that a conserved mechanism applies to the development of blood vessels in all coelomic organs.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

October 28, 2008

Volume

105

Issue

43

Start / End Page

16626 / 16630

Location

United States

Related Subject Headings

  • Receptor, Platelet-Derived Growth Factor beta
  • Organogenesis
  • Muscle, Smooth, Vascular
  • Mice
  • Mesoderm
  • Lung
  • Genes, Reporter
  • Fluorescent Dyes
  • Epithelium
  • Embryo, Mammalian
 

Citation

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Que, J., Wilm, B., Hasegawa, H., Wang, F., Bader, D., & Hogan, B. L. M. (2008). Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development. Proc Natl Acad Sci U S A, 105(43), 16626–16630. https://doi.org/10.1073/pnas.0808649105
Que, Jianwen, Bettina Wilm, Hiroshi Hasegawa, Fan Wang, David Bader, and Brigid L. M. Hogan. “Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development.Proc Natl Acad Sci U S A 105, no. 43 (October 28, 2008): 16626–30. https://doi.org/10.1073/pnas.0808649105.
Que J, Wilm B, Hasegawa H, Wang F, Bader D, Hogan BLM. Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development. Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16626–30.
Que, Jianwen, et al. “Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development.Proc Natl Acad Sci U S A, vol. 105, no. 43, Oct. 2008, pp. 16626–30. Pubmed, doi:10.1073/pnas.0808649105.
Que J, Wilm B, Hasegawa H, Wang F, Bader D, Hogan BLM. Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development. Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16626–16630.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

October 28, 2008

Volume

105

Issue

43

Start / End Page

16626 / 16630

Location

United States

Related Subject Headings

  • Receptor, Platelet-Derived Growth Factor beta
  • Organogenesis
  • Muscle, Smooth, Vascular
  • Mice
  • Mesoderm
  • Lung
  • Genes, Reporter
  • Fluorescent Dyes
  • Epithelium
  • Embryo, Mammalian