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Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.

Publication ,  Journal Article
Lemaire, K; Moura, RF; Granvik, M; Igoillo-Esteve, M; Hohmeier, HE; Hendrickx, N; Newgard, CB; Waelkens, E; Cnop, M; Schuit, F
Published in: PLoS One
April 6, 2011

UFM1 is a member of the ubiquitin like protein family. While the enzymatic cascade of UFM1 conjugation has been elucidated in recent years, the biological function remains largely unknown. In this report we demonstrate that the recently identified C20orf116, which we name UFM1-binding protein 1 containing a PCI domain (UFBP1), and CDK5RAP3 interact with UFM1. Components of the UFM1 conjugation pathway (UFM1, UFBP1, UFL1 and CDK5RAP3) are highly expressed in pancreatic islets of Langerhans and some other secretory tissues. Co-localization of UFM1 with UFBP1 in the endoplasmic reticulum (ER) depends on UFBP1. We demonstrate that ER stress, which is common in secretory cells, induces expression of Ufm1, Ufbp1 and Ufl1 in the beta-cell line INS-1E. siRNA-mediated Ufm1 or Ufbp1 knockdown enhances apoptosis upon ER stress. Silencing the E3 enzyme UFL1, results in similar outcomes, suggesting that UFM1-UFBP1 conjugation is required to prevent ER stress-induced apoptosis. Together, our data suggest that UFM1-UFBP1 participate in preventing ER stress-induced apoptosis in protein secretory cells.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

April 6, 2011

Volume

6

Issue

4

Start / End Page

e18517

Location

United States

Related Subject Headings

  • Stress, Physiological
  • RNA, Messenger
  • Proteins
  • Protein Transport
  • Protein Binding
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry
 

Citation

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Lemaire, K., Moura, R. F., Granvik, M., Igoillo-Esteve, M., Hohmeier, H. E., Hendrickx, N., … Schuit, F. (2011). Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis. PLoS One, 6(4), e18517. https://doi.org/10.1371/journal.pone.0018517
Lemaire, Katleen, Rodrigo F. Moura, Mikaela Granvik, Mariana Igoillo-Esteve, Hans E. Hohmeier, Nico Hendrickx, Christopher B. Newgard, Etienne Waelkens, Miriam Cnop, and Frans Schuit. “Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.PLoS One 6, no. 4 (April 6, 2011): e18517. https://doi.org/10.1371/journal.pone.0018517.
Lemaire K, Moura RF, Granvik M, Igoillo-Esteve M, Hohmeier HE, Hendrickx N, et al. Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis. PLoS One. 2011 Apr 6;6(4):e18517.
Lemaire, Katleen, et al. “Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.PLoS One, vol. 6, no. 4, Apr. 2011, p. e18517. Pubmed, doi:10.1371/journal.pone.0018517.
Lemaire K, Moura RF, Granvik M, Igoillo-Esteve M, Hohmeier HE, Hendrickx N, Newgard CB, Waelkens E, Cnop M, Schuit F. Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis. PLoS One. 2011 Apr 6;6(4):e18517.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

April 6, 2011

Volume

6

Issue

4

Start / End Page

e18517

Location

United States

Related Subject Headings

  • Stress, Physiological
  • RNA, Messenger
  • Proteins
  • Protein Transport
  • Protein Binding
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry