An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses
Publication
, Journal Article
An, Y; Stevens, RD; Muenzer, J; Clarke, JTR; Millington, DS
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
A quantitative liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) is developed to measure the characterstic disaccharide derived from glycosaminoglycans (GAG) by partial methanolysis. The results show that methanolysis replace the sulphate groups by hydroxyl groups and methylates. The polymeric GAG collapse to a common disaccharide, the sodiated adduct of which dissociates to a monosaccharide by CID. The measured GAG concentrations were normalized by urinary creatinine content.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
597 / 598
Citation
APA
Chicago
ICMJE
MLA
NLM
An, Y., Stevens, R. D., Muenzer, J., Clarke, J. T. R., & Millington, D. S. (2002). An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 597–598.
An, Y., R. D. Stevens, J. Muenzer, J. T. R. Clarke, and D. S. Millington. “An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 597–98.
An Y, Stevens RD, Muenzer J, Clarke JTR, Millington DS. An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;597–8.
An, Y., et al. “An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 597–98.
An Y, Stevens RD, Muenzer J, Clarke JTR, Millington DS. An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;597–598.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
597 / 598